Transmission Electron Microscopy: Theory and Applications
Pp. 96-116 (21)
Transmission Electron Microscopy (TEM) is a useful technique to explore
the molecular structure, interactions and processes including structure-function
relationships at cellular level using a variety of TEM techniques with resolution of one
angstrom (Ǻ) 1 to 1000 Ǻ. Developments in modern science and technology, especially
in the material science, depend significantly on microstructure characterization. In this
context, novel characterization techniques are crucial to understand the properties of
materials. The quality of TEM results is dependent on preparation of TEM grid.
Experts are constantly working on optimization of milling parameters to reduce the
potential artifacts. The resolution power of TEM makes it possible to visualize different
objects with high quality images to study complex structures and tissue morphology.
Thus, TEM has made a milestone towards the understating of cellular structure. This
chapter includes introduction and theory of TEM including its instrumentation, sample
preparation, and working applications.
Backscattered electron, Instrumentation, Sample preparation,
Transmission electron microscope.