Essential Techniques for Medical and Life Scientists: A Guide to Contemporary Methods and Current Applications- Part II

Essential Techniques for Medical and Life Scientists: A Guide to Contemporary Methods and Current Applications- Part II

This handbook covers some primary instruments-based techniques used in modern biological science and medical research programs. Key features of the book include introductory notes for each topic, a ...
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Circular Dichroism

Pp. 85-95 (11)

DOI: 10.2174/9789811464867120010007

Author(s): Yusuf Tutar*

Abstract

Circular Dichroism (CD) is an absorption spectroscopy technique and measures left and right circularly polarized light difference of optical activity of asymmetric macromolecules. Unequal absorption of left and right circularly polarized light provides information about macromolecules depending on the spectral region. The far UV region provides data for secondary structure composition of proteins whereas near UV region provides data for tertiary structure of proteins. Furthermore, denaturation experiments provide stability parameters of proteins.

Keywords:

Circular Dichroism, Far UV region, Folding, Kinetic measurements, Near UV region, Stability.