Pp. 85-95 (11)
Circular Dichroism (CD) is an absorption spectroscopy technique and
measures left and right circularly polarized light difference of optical activity of
asymmetric macromolecules. Unequal absorption of left and right circularly polarized
light provides information about macromolecules depending on the spectral region. The
far UV region provides data for secondary structure composition of proteins whereas
near UV region provides data for tertiary structure of proteins. Furthermore,
denaturation experiments provide stability parameters of proteins.
Circular Dichroism, Far UV region, Folding, Kinetic measurements,
Near UV region, Stability.