Aim and Objective: In ancient China, rice bran was used to treat diabetes and hyperlipidemia. The aim of this paper is to explore the active compounds and underlying mechanism of rice bran petroleum ether extracts (RBPE) against diabetes using network pharmacology.
Materials and Methods: Gas chromatography-mass spectrometeranalysis was performed to identify the chemical composition in RBPE. Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform, Swiss Target Prediction database, BATMAN-TCM, comprehensive database of human genes and gene phenotypes, therapeutic target database,
DurgBank and GeneCards database were used to screen targets. The "component-target-disease" interactive network was
constructed by Cytoscape software. Gene ontology and pathways related to the targets were analyzed by ClueGO and core
targets were screened by the MCODE, and Autodock vina was used for molecular docking.
Results: The compounds with a percentage greater than 1.0% was selected for subsequent analysis. The RBPE contains oleic acid, (E)-9-Octadecenoic acid ethyl ester, and other chemical components that can regulate insulin, mitogen-activated
protein kinase 3, epidermal growth factor receptor, mitogen-activated protein kinase 1, and other genes, which were mainly
related to Pathways in cancer, Human cytomegalovirus infection and AGE-RAGE signaling pathway in diabetic complications, etc. The affinity of the core compounds and the corresponding protein of the gene targets was good.
Conclusion: The results of network pharmacology analysis indicate that the RBPE has multiple anti-diabetic ingredients,
and RBPE exert anti-diabetic activity through multiple targets and signaling pathways. Present study can provide a scientific
basis for further elucidating the mechanism of RBPE against diabetes.