Background: Tylophora hirsuta (Wall) has long been used as traditional medicine for
the treatment of diabetes. The current study is designed to evaluate the anti-diabetic and anti-inflammatory
activity of different extracts of aerial parts of Tylophora hirsuta.
Methods: Sequential maceration was conducted to obtain extracts. Total phenolic contents were determined
by the Folin-Ciocalteau method. The anti-oxidant activity was assessed by DPPH free radical
scavenging assay. The extracts were tested for its inhibitory activity against α-amylase in-vitro.
In-vivo anti-diabetic assay was conducted using alloxan-induced diabetic model and OGTT was
conducted on normal rats. ELISA was used to determine the pro-inflammatory cytokines (TNF-α
and IL-6). The polyphenolic composition of the extract was analyzed using an HPLC system.
Results: Aqueous extract exhibited highest total phenolic contents (985.24± 3.82 mg GAE/100 g
DW), antioxidant activity (IC50 = 786.70 ± 5.23 μg/mL), and alpha-amylase inhibition (IC50
=352.8 μg/mL). The aqueous extract of Tylophora hirsuta showed remarkable in-vivo anti-diabetic
activity. Results were compared with standard drug glibenclamide. Alloxan induced diabetic mediated
alterations in liver function enzymes, renal function determinants, and lipid parameters were
significantly restored in aqueous extract treated diabetic rats. A significant reduction in pro-inflammatory
cytokines (p<0.001) was observed when compared to the control group. HPLC analysis confirms
the presence of quercetin, gallic acid, cinnamic acid, and p-coumaric acid.
Conclusion: These results showed that Tylophora hirsuta possesses strong anti-diabetic and anti-inflammatory
potentials and justify its folklore use for the management of diabetes.