Background: Natural products constitute more than half of all biomolecules lately being
used in clinical settings. Mannoprotein derived from the yeast cell wall has found full biotechnological
Objective: This study was intended to investigate the antioxidant, anticancer, and toxicological
properties of Kluyveromyces marxianus mannoprotein (KM).
Methods: The KM extract was obtained through a sequence of operations, including centrifugation
for cell isolation, precipitation with potassium citrate/sodium metabisulfite, and recovery and purification.
Its antioxidant, growth inhibition, macrophage mitogenic, and toxic activities were evaluated
for its future use in the biomedical field.
Results: Significant inhibitory effects of KM were obtained on reactive species. It showed antiproliferative
activity against HeLa (human cervical adenocarcinoma) and MCF-7 (human breast cancer)
cell lines with no toxic effects on HUVECs (human umbilical vein endothelial cells). The in
vitro model of CHO-K1 (Chinese hamster ovary) cell lines did not show the cytotoxic and genotoxic
of KM. Moreover, it enhanced macrophage activity in terms of nitric oxide (NO) production and
viability. No sign of acute toxicity was found in BALB/c mice, and body weight remained
unchanged in guinea pigs over three months.
Conclusion: Comprehensive biological evaluations in this study are expected to expand the potential
of KM as a natural material.