Evaluation of Prophylactic and Therapeutic Roles of N-Acetylcysteine on Biochemical and Oxidative Changes Induced by Acute Poisoning of Diazinon in Various Rat Tissues

Author(s): Kavoos Tahmasebi, Mahvash Jafari*, Farideh Izadi, Alireza Asgari, Hoosein Bahadoran, Javad Heydari, Saeed Khazaie

Journal Name: Current Chemical Biology

Volume 14 , Issue 2 , 2020

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Graphical Abstract:


Background: Exposure to diazinon (DZN) as an organophosphorus insecticide is associated with reducing the antioxidant capacity of cells. N-acetyl cysteine (NAC) is widely used in clinics to treat several diseases related to oxidative stress.

Objective: The current study was aimed to evaluate the prophylactic and therapeutic roles of NAC on biochemical and oxidative changes induced by acute poisoning of DZN in various tissues of male Wistar rats.

Methods: Thirty rats were divided into five groups: control group received corn oil as DZN solvent; DZN group received 100 mg/kg of DZN; NAC group received 160 mg/kg of NAC; NAC-DZN and DZN-NAC groups received 160 mg/kg of NAC before and after 100 mg/kg of DZN injection, respectively. Plasma and various tissues were prepared and evaluated for the measurement of the biochemical parameters and oxidative stress biomarkers.

Results: Both prophylactic and therapeutic treatments by NAC ameliorated the increased lipid peroxidation and decreased glutathione level and superoxide dismutase, catalase and glutathione S-transferase activities in tissues (P<0.05). Moreover, treatment with the NAC caused a significant reduction in DZN-induced high levels of plasma biochemical parameters. Furthermore, acetylcholinesterase activity was positively correlated with both LDH (P=0.000) activity and GSH (P=0.001) level and negatively correlated with MDA (P=0.009) level in the brain.

Conclusion: Results suggest that NAC could effectively ameliorate the DZN-induced oxidative stress and cholinergic hyperactivity in various tissues especially in the brain, through free radicals scavenging and GSH synthesis. Prophylactic approach exerted a stronger protective effect compared to a therapeutic treatment.

Keywords: Diazinon, N-acetylcysteine, oxidative stress, acetylcholinesterase, biochemical parameters, tissues, rat.

El-Shenawy NS, El-Salmy F, Al-Eisa RA, El-Ahmary B. Amelioratory effect of vitamin E on organophosphorus insecticide diazinon-induced oxidative stress in mice liver. Pestic Biochem Physiol 2010; 96: 101-7.
Pirsaheb M, Fattahi N, Rahimi R, Sharafi K, Ghaffari HR. Evaluation of abamectin, diazinon and chlorpyrifos pesticide residues in apple product of Mahabad region gardens: Iran in 2014. Food Chem 2017; 231: 148-55.
[http://dx.doi.org/10.1016/j.foodchem.2017.03.120] [PMID: 28449991]
Khazaie S, Jafari M, Heydari J, et al. Modulatory effects of vitamin C on biochemical and oxidative changes induced by acute exposure to diazinon in rat various tissues: Prophylactic and therapeutic roles. J Anim Physiol Anim Nutr (Berl) 2019; 103(5): 1619-28.
[http://dx.doi.org/10.1111/jpn.13144] [PMID: 31218763]
Beydilli H, Yilmaz N, Cetin ES, et al. Evaluation of the protective effect of silibinin against diazinon induced hepatotoxicity and free-radical damage in rat liver. Iran Red Crescent Med J 2015; 17(4)e25310
[http://dx.doi.org/10.5812/ircmj.17(4)2015.25310] [PMID: 26023342]
Ajibade TO, Oyagbemi AA, Omobowale TO, Asenuga ER, Afolabi JM, Adedapo AA. Mitigation of diazinon-induced cardiovascular and renal dysfunction by gallic acid. Interdiscip Toxicol 2016; 9(2): 66-77.
[http://dx.doi.org/10.1515/intox-2016-0008] [PMID: 28652848]
Jafari M, Salehi M, Ahmadi S, Asgari A, Abasnezhad M, Hajigholamali M. The role of oxidative stress in diazinon-induced tissues toxicity in Wistar and Norway rats. Toxicol Mech Methods 2012; 22(8): 638-47.
[http://dx.doi.org/10.3109/15376516.2012.716090] [PMID: 22871176]
John J, Gujar N, Prasad G, Bhattacharya R. Effect of N-acetylcysteine on time- dependent biochemical and oxidative changes after acute diisopropyl phosphorofluoridate poisoning in mice. Inter J Pharm Res 2017; 7: 43-54.
Karami-Mohajeri S, Ahmadipour A, Rahimi H-R, Abdollahi M. Adverse effects of organophosphorus pesticides on the liver: a brief summary of four decades of research. Arh Hig Rada Toksikol 2017; 68(4): 261-75.
[http://dx.doi.org/10.1515/aiht-2017-68-2989] [PMID: 29337682]
Yu F, Wang Z, Ju B, Wang Y, Wang J, Bai D. Apoptotic effect of organophosphorus insecticide chlorpyrifos on mouse retina in vivo via oxidative stress and protection of combination of vitamins C and E. Exp Toxicol Pathol 2008; 59(6): 415-23.
[http://dx.doi.org/10.1016/j.etp.2007.11.007] [PMID: 18222074]
Yurumez Y, Cemek M, Yavuz Y, Birdane YO, Buyukokuroglu ME. Beneficial effect of N-acetylcysteine against organophosphate toxicity in mice. Biol Pharm Bull 2007; 30(3): 490-4.
[http://dx.doi.org/10.1248/bpb.30.490] [PMID: 17329844]
Mostafalou S, Abdollahi M, Eghbal MA, Saeedi Kouzehkonani N. Protective effect of NAC against malathion-induced oxidative stress in freshly isolated rat hepatocytes. Adv Pharm Bull 2012; 2(1): 79-88.
[PMID: 24312774]
Oksay T, Nazıroğlu M, Ergün O, et al. N-acetyl cysteine attenuates diazinon exposure-induced oxidative stress in rat testis. Andrologia 2013; 45(3): 171-7.
[http://dx.doi.org/10.1111/j.1439-0272.2012.01329.x] [PMID: 22742659]
Jafari M, Salehi M, Zardooz H, Rostamkhani F. Response of liver antioxidant defense system to acute and chronic physical and psychological stresses in male rats. EXCLI J 2014; 13: 161-71.
[PMID: 26417250]
Dessouki AA, Hassan AG, Ali SA, Loutfy NM, Ahmed MT. Alterations in P53 gene, testicular and hepatic tissues of albino rats due to profenofos administration: A Possible protective effect of vitamin C. Immunol Toxicol 2013; 1: 26-34.
Shadnia S, Dasgar M, Taghikhani S, Mohammadirad A, Khorasani R, Abdollahi M. Protective effects of α-tocopherol and N-acetyl-cysteine on diazinon-induced oxidative stress and acetylcholinesterase inhibition in rats. Toxicol Mech Methods 2007; 17(2): 109-15.
[http://dx.doi.org/10.1080/15376510600860318] [PMID: 20020979]
Uner N, Sevgiler Y, Durmaz H, Piner P, Cinkiloğlu E. N-Acetylcysteine provides dose-dependent protection against fenthion toxicity in the brain of Cyprinus carpio L. Comp Biochem Physiol C Toxicol Pharmacol 2009; 150(1): 33-8.
[http://dx.doi.org/10.1016/j.cbpc.2009.02.001] [PMID: 19444991]
Cankayali I, Demirag K, Eris O, Ersoz B, Moral AR. The effects of N-acetylcysteine on oxidative stress in organophosphate poisoning model. Adv Ther 2005; 22(2): 107-16.
[http://dx.doi.org/10.1007/BF02849882] [PMID: 16020401]
Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 1972; 18(6): 499-502.
[http://dx.doi.org/10.1093/clinchem/18.6.499] [PMID: 4337382]
Winterbourn CC, Hawkins RE, Brian M, Carrell RW. The estimation of red cell superoxide dismutase activity. J Lab Clin Med 1975; 85(2): 337-41.
[PMID: 803541]
Aebi H. Catalase in vitro. Methods Enzymol 1984; 105: 121-6.
[http://dx.doi.org/10.1016/S0076-6879(84)05016-3] [PMID: 6727660]
Habig WH, Jakoby WB. Glutathione S-transferases (rat and human). Methods Enzymol 1981; 77: 218-31.
[http://dx.doi.org/10.1016/S0076-6879(81)77029-0] [PMID: 6173570]
Tietze F. Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione: applications to mammalian blood and other tissues. Anal Biochem 1969; 27(3): 502-22.
[http://dx.doi.org/10.1016/0003-2697(69)90064-5] [PMID: 4388022]
Satoh K. Serum lipid peroxide in cerebrovascular disorders determined by a new colorimetric method. Clin Chim Acta 1978; 90(1): 37-43.
[http://dx.doi.org/10.1016/0009-8981(78)90081-5] [PMID: 719890]
Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976; 72: 248-54.
[http://dx.doi.org/10.1016/0003-2697(76)90527-3] [PMID: 942051]
Van Kampen E, Zijlstra WG. Determination of hemoglobin and its derivatives Advances in Clinical Chemistry. Elsevier 1966; pp. 141-87.
Dean O, Giorlando F, Berk M. N-acetylcysteine in psychiatry: current therapeutic evidence and potential mechanisms of action. J Psychiatry Neurosci 2011; 36(2): 78-86.
[http://dx.doi.org/10.1503/jpn.100057] [PMID: 21118657]
Dodd S, Dean O, Copolov DL, Malhi GS, Berk M. N-acetylcysteine for antioxidant therapy: pharmacology and clinical utility. Expert Opin Biol Ther 2008; 8(12): 1955-62.
[http://dx.doi.org/10.1517/14728220802517901] [PMID: 18990082]
de Andrade KQ, Moura FA, dos Santos JM, de Araújo ORP, de Farias Santos JC, Goulart MOF. Oxidative stress and inflammation in hepatic diseases: therapeutic possibilities of N-acetylcysteine. Int J Mol Sci 2015; 16(12): 30269-308.
[http://dx.doi.org/10.3390/ijms161226225] [PMID: 26694382]
Turner PV, Brabb T, Pekow C, Vasbinder MA. Administration of substances to laboratory animals: routes of administration and factors to consider. J Am Assoc Lab Anim Sci 2011; 50(5): 600-13.
[PMID: 22330705]
Correa MJU, Mariz HA, Andrade LEC, Kayser C. Oral N-acetylcysteine in the treatment of Raynaud’s phenomenon secondary to systemic sclerosis: a randomized, double-blind, placebo-controlled clinical trial. Rev Bras Reumatol 2014; 54(6): 452-8.
[http://dx.doi.org/10.1016/j.rbr.2014.07.001] [PMID: 25458027]
Deng X, Xia Y, Hu W, Zhang H, Shen Z. Cadmium-induced oxidative damage and protective effects of N-acetyl-L-cysteine against cadmium toxicity in Solanum nigrum L. J Hazard Mater 2010; 180(1-3): 722-9.
[http://dx.doi.org/10.1016/j.jhazmat.2010.04.099] [PMID: 20488618]
Aldini G, Altomare A, Baron G, et al. N-Acetylcysteine as an antioxidant and disulphide breaking agent: the reasons why. Free Radic Res 2018; 52(7): 751-62.
[http://dx.doi.org/10.1080/10715762.2018.1468564] [PMID: 29742938]
Mokhtari V, Afsharian P, Shahhoseini M, Kalantar SM, Moini A. A review on various uses of N-acetyl cysteine. Cell J 2017; 19(1): 11-7.
[PMID: 28367412]
Machado JT, Iborra RT, Fusco FB, et al. N-acetylcysteine prevents endoplasmic reticulum stress elicited in macrophages by serum albumin drawn from chronic kidney disease rats and selectively affects lipid transporters, ABCA-1 and ABCG-1. Atherosclerosis 2014; 237(1): 343-52.
[http://dx.doi.org/10.1016/j.atherosclerosis.2014.09.020] [PMID: 25305669]
Van Hecke O, Lee J. N-acetylcysteine: A rapid review of the evidence for effectiveness in treating COVID-19 Centre for Evidence-Based Medicine. Oxford University 2020.
Peña-Llopis S, Ferrando MD, Peña JB. Fish tolerance to organophosphate-induced oxidative stress is dependent on the glutathione metabolism and enhanced by N-acetylcysteine. Aquat Toxicol 2003; 65(4): 337-60.
[http://dx.doi.org/10.1016/S0166-445X(03)00148-6] [PMID: 14568351]
Shadnia S, Ashrafivand S, Mostafalou S, Abdollahi M. N-acetylcysteine a novel treatment for acute human organophosphate poisoning. Int J Pharmacol 2011; 7: 732-5.
Mansour SA-K, Abbassy MA-L, Shaldam HA. Hepato- Renal Toxicity Induced by Chlorpyrifos, Diazinon and their Mixture to Male Rats with Special Concern to the Effect of Zinc Supplementation J Toxicol Pharmacol 2017.
Al-Shinnawy MA. Vitamin “C” as ameliorative agent against thiodicarb toxicated male albino rats (Rattus norvegicus). Egypt Acad J Biol Sci 2008; 1: 177-87.
Jafari M, Salehi M, Asgari A, et al. Effects of paraoxon on serum biochemical parameters and oxidative stress induction in various tissues of Wistar and Norway rats. Environ Toxicol Pharmacol 2012; 34(3): 876-87.
[http://dx.doi.org/10.1016/j.etap.2012.08.011] [PMID: 23021855]
Messarah M, Amamra W, Boumendjel A, et al. Ameliorating effects of curcumin and vitamin E on diazinon-induced oxidative damage in rat liver and erythrocytes. Toxicol Ind Health 2013; 29(1): 77-88.
[http://dx.doi.org/10.1177/0748233712446726] [PMID: 22609857]
Ambali SF, Akanbi DO, Shittu M, Giwa A, Oladipo O, Ayo J. Chlorpyrifos-induced clinical, haematological and biochemical changes in Swiss albino mice: mitigating effect by co-administration of vitamins C and E. Life Sci J 2010; 7: 37-44.
Ambali SF, Onukak C, Idris SB, Yaqub LS. Vitamin C attenuates short-term hematological and biochemical alterations induced by acute chlorpyrifos exposure in Wistar rats. J Med Med Sci 2010; 1: 465-77.
Lahmass I, Ouahhoud S, Elyoubi M, Benabbas R, Sabouni A, Asehraou A, et al. Evaluation of antioxidant activities of saffron stigma and spath as by-product of Crocus sativus L. MOJ. Biol Med (Aligarh) 2018; 3: 154-8.
Abu-Sheir WA. The Protective Role of Vitamin C against Histological Changes and Some Biochemical Indices in Liver of Rats Chronically Exposed to Diazinon. Egypt J Hosp Med 2016; 64: 395-404.
Al-Attar AM, Al-Taisan W. Preventive effects of black seed (Nigella sativa) extract on Sprague Dawley rats exposed to diazinon. Australian J Appl Sci 2010; 4: 957-68.
Banaee M, Sureda A, Mirvaghefi A, Ahmadi K. Effects of diazinon on biochemical parameters of blood in rainbow trout (Oncorhynchus mykiss). Pestic Biochem Physiol 2011; 99: 1-6.
El-Demerdash FM. Lipid peroxidation, oxidative stress and acetylcholinesterase in rat brain exposed to organophosphate and pyrethroid insecticides. Food Chem Toxicol 2011; 49(6): 1346-52.
[http://dx.doi.org/10.1016/j.fct.2011.03.018] [PMID: 21419823]
Salehi M, Jafari M, Asgari A, Salimian M, Abbasnezhad M, Hosseini RH, et al. Strain-related differences on response of liver and kidney antioxidant defense system in two rat strains following diazinon exposure Zahedan J Res Med Sci 2016. 18e5988
Al-Attar AM, Elnaggar MHR, Almalki EA. Protective effect of some plant oils on diazinon induced hepatorenal toxicity in male rats. Saudi J Biol Sci 2017; 24(6): 1162-71.
[http://dx.doi.org/10.1016/j.sjbs.2016.10.013] [PMID: 28855808]
Zainab WK, Salman HA, Ebraheem MK. Histopathological changes that induced in the internal organs of white rat after exposure to diazinon. Bas J Vet Res 2017; 16: 223-39.
Tahmasebi K, Jafari M, Izadi F. Study of the protective effect of N-acetyl cysteine against acute diazinon-induced oxidative stress in rat brain and heart. J Ardabil Univ Med Sci 2015; 15: 116-27. [Persian].
Vahidirad M, Arab-Nozari M, Mohammadi H, Zamani E, Shaki F. Protective effect of captopril against diazinon induced nephrotoxicity and neurotoxicity via inhibition of ROS-NO pathway. Drug Chem Toxicol 2018; 41(3): 287-93.
[http://dx.doi.org/10.1080/01480545.2017.1391830] [PMID: 29115169]
Beceren A, Sehirli AO, Omurtag GZ, Arbak S, Turan P, Sener G. Protective effect of N-Acetyl-L-Cysteine (NAC) on endosulfan-induced liver and kidney toxicity in rats. Int J Clin Exp Med 2017; 10: 10031-9.
Lasram MM, Bini Douib I, Bouzid K, et al. Effects of N-acetyl-l-cysteine, in vivo, against pathological changes induced by malathion. Toxicol Mech Methods 2014; 24(4): 294-306.
[http://dx.doi.org/10.3109/15376516.2014.886003] [PMID: 24785381]
El-Bini Dhouib I, Annabi A, Jrad A, et al. Carbosulfan-induced oxidative damage following subchronic exposure and the protective effects of N-acetylcysteine in rats. Gen Physiol Biophys 2015; 34(3): 249-61.
[http://dx.doi.org/10.4149/gpb_2015005] [PMID: 25926552]
Rezg R, Mornagui B, El-Fazaa S, Gharbi N. Caffeic acid attenuates malathion induced metabolic disruption in rat liver, involvement of acetylcholinesterase activity. Toxicology 2008; 250(1): 27-31.
[http://dx.doi.org/10.1016/j.tox.2008.05.017] [PMID: 18588939]
Lasram MM, El-Golli N, Lamine AJ, et al. Changes in glucose metabolism and reversion of genes expression in the liver of insulin-resistant rats exposed to malathion. The protective effects of N-acetylcysteine. Gen Comp Endocrinol 2015; 215: 88-97.
[http://dx.doi.org/10.1016/j.ygcen.2014.10.002] [PMID: 25449180]
Pourkhalili N, Pournourmohammadi S, Rahimi F, et al. Comparative effects of calcium channel blockers, autonomic nervous system blockers, and free radical scavengers on diazinon-induced hyposecretion of insulin from isolated islets of Langerhans in rats. Arh Hig Rada Toksikol 2009; 60(2): 157-64.
[http://dx.doi.org/10.2478/10004-1254-60-2009-1917] [PMID: 19581208]
Boroushaki MT, Arshadi D, Jalili-Rasti H, Asadpour E, Hosseini A. Protective effect of pomegranate seed oil against acute toxicity of diazinon in rat kidney. Iran J Pharm Res 2013; 12(4): 821-7.
[PMID: 24523762]
Bhatti GK, Bhatti JS, Kiran R, Sandhir R. Alterations in Ca2+ homeostasis and oxidative damage induced by ethion in erythrocytes of Wistar rats: ameliorative effect of vitamin E. Environ Toxicol Pharmacol 2011; 31(3): 378-86.
[http://dx.doi.org/10.1016/j.etap.2011.01.004] [PMID: 21787708]
Falach-Malik A, Rozenfeld H, Chetboun M, et al. N-Acetyl-L-Cysteine inhibits the development of glucose intolerance and hepatic steatosis in diabetes-prone mice. Am J Transl Res 2016; 8(9): 3744-56.
[PMID: 27725855]
Tanko Y, Eze E, Chukwuemeka U, Jimoh A, Mohammed K, Abdulrazak A, et al. Modulatory roles of vitamin C and E on blood glucose and serum electrolytes levels in fructose-induced insulin resistance (Type 2) diabetes mellitus in wistar rats. Pharm Lett 2013; 5: 259-63.
Elmonem HA. Assessment the effect of pomegranate molasses against diazinon toxicity in male Rats. J Environ Sci Toxicol Food Technol 2014; 8: 135-41.
Al-Attar AM. Effect of grapeseed oil on diazinon-induced physiological and histopathological alterations in rats. Saudi J Biol Sci 2015; 22(3): 284-92.
[http://dx.doi.org/10.1016/j.sjbs.2014.12.010] [PMID: 25972749]
Ibrahim ATA. Protective role of lycopene and vitamin E against diazinon-induced biochemical changes in Oreochromis niloticus. Afr J Environ Sci Technol 2015; 9: 557-65.
Abedini MS, Jafari M, Mirzadeh SM, Salem F. The effect of N-acetyl cysteine and vitamins E and C on diazinon-induced oxidative stress in rat erythrocytes. Daneshvar Medicine 2016; 23: 53-63. [Persian].
Lee JE, Lim MS, Park JH, Park CH, Koh HC. Nuclear NF-κB contributes to chlorpyrifos-induced apoptosis through p53 signaling in human neural precursor cells. Neurotoxicology 2014; 42: 58-70.
[http://dx.doi.org/10.1016/j.neuro.2014.04.001] [PMID: 24727577]
Slotkin TA, Seidler FJ. Oxidative and excitatory mechanisms of developmental neurotoxicity: transcriptional profiles for chlorpyrifos, diazinon, dieldrin, and divalent nickel in PC12 cells. Environ Health Perspect 2009; 117(4): 587-96.
[http://dx.doi.org/10.1289/ehp.0800251] [PMID: 19440498]
Surajudeen YA, Sheu RK, Ayokulehin KM, Olatunbosun AG. Oxidative stress indices in Nigerian pesticide applicators and farmers occupationally exposed to organophosphate pesticides. Int J Appl Basic Med Res 2014; 4(Suppl. 1): S37-40.
[http://dx.doi.org/10.4103/2229-516X.140730] [PMID: 25298941]
Ahmadi S, Jafari M, Asgari A, Salehi M. Acute effect of diazinon on the antioxidant system of rat’s heart tissue. Trauma Mon 2011; 2011: 87-93. [Persian].
Oruc E. Effects of diazinon on antioxidant defense system and lipid peroxidation in the liver of Cyprinus carpio (L.). Environ Toxicol 2011; 26(6): 571-8.
[http://dx.doi.org/10.1002/tox.20573] [PMID: 20196151]
Shah MD, Iqbal M. Diazinon-induced oxidative stress and renal dysfunction in rats. Food Chem Toxicol 2010; 48(12): 3345-53.
[http://dx.doi.org/10.1016/j.fct.2010.09.003] [PMID: 20828599]
Yilmaz N, Yilmaz M, Altuntas I. Diazinon-induced brain toxicity and protection by vitamins E plus C. Toxicol Ind Health 2012; 28(1): 51-7.
[http://dx.doi.org/10.1177/0748233711404035] [PMID: 21543467]
Samuni Y, Goldstein S, Dean OM, Berk M. The chemistry and biological activities of N-acetylcysteine. Biochim Biophys Acta 2013; 1830(8): 4117-29.
[http://dx.doi.org/10.1016/j.bbagen.2013.04.016] [PMID: 23618697]
Asevedo E, Mendes AC, Berk M, Brietzke E. Systematic review of N-acetylcysteine in the treatment of addictions. Br J Psychiatry 2014; 36(2): 168-75.
[http://dx.doi.org/10.1590/1516-4446-2013-1244] [PMID: 24676047]
Pei Y, Liu H, Yang Y, et al. Biological activities and potential oral applications of N-acetylcysteine: progress and prospects. Oxid Med Cell Longev 2018.20182835787
[http://dx.doi.org/10.1155/2018/2835787] [PMID: 29849877]
Agrahari S, Pandey KC, Gopal K. Biochemical alteration induced by monocrotophos in the blood plasma of fish, Channa punctatus (Bloch). Pestic Biochem Physiol 2007; 88: 268-72.
Fishbane S. N-acetylcysteine in the prevention of contrast-induced nephropathy. Clin J Am Soc Nephrol 2008; 3(1): 281-7.
[http://dx.doi.org/10.2215/CJN.02590607] [PMID: 18003766]
Dorval J, Hontela A. Role of glutathione redox cycle and catalase in defense against oxidative stress induced by endosulfan in adrenocortical cells of rainbow trout (Oncorhynchus mykiss). Toxicol Appl Pharmacol 2003; 192(2): 191-200.
[http://dx.doi.org/10.1016/S0041-008X(03)00281-3] [PMID: 14550752]
Sevgiler Y, Piner P, Durmaz H, Uner N. Effects of N-acetylcysteine on oxidative responses in the liver of fenthion exposed Cyprinus carpio. Pestic Biochem Physiol 2007; 87: 248-54.
Izadi F, Jafari M, Bahdoran H, Asgari A, Divsalar A, Salehi M. The role of N-acetyl cysteine on reduction of diazinon-induced oxidative stress in rat liver and kidney. Majallah-i Ilmi-i Danishgah-i Ulum-i Pizishki-i Rafsanjan 2014; 12: 895-906. [Persian].
Heydari J, Jafari M, Khazaie S. Comparison of antioxidant properties of N-acetylcysteine and vitamins E and C on diazinon-induced oxidative stress in rat spleen. J Birjand Univ Med Sci 2016; 23: 101-9. [Persian].
Mousavi SR, Jafari M, Rezaei S, Agha-Alinejad H, Sobhani V. Evaluation of the effects of different intensities of forced running wheel exercise on oxidative stress biomarkers in muscle, liver and serum of untrained rats. Lab Anim (NY) 2020; 49(4): 119-25.
[http://dx.doi.org/10.1038/s41684-020-0503-7] [PMID: 32203319]
Shen H, Li M, Wang B, Lai IK, Robertson LW, Ludewig G. Dietary antioxidants (selenium and N-acetylcysteine) modulate paraoxonase 1 (PON1) in PCB 126-exposed rats. Environ Sci Pollut Res Int 2014; 21(10): 6384-99.
[http://dx.doi.org/10.1007/s11356-013-1690-1] [PMID: 23644946]
Balszuweit F, Menacher G, Schmidt A, et al. Protective effects of the thiol compounds GSH and NAC against sulfur mustard toxicity in a human keratinocyte cell line. Toxicol Lett 2016; 244: 35-43.
[http://dx.doi.org/10.1016/j.toxlet.2015.09.002] [PMID: 26361990]
Bavarsad Shahripour R, Harrigan MR, Alexandrov AV. N-acetylcysteine (NAC) in neurological disorders: mechanisms of action and therapeutic opportunities. Brain Behav 2014; 4(2): 108-22.
[http://dx.doi.org/10.1002/brb3.208] [PMID: 24683506]
Ogutcu A, Uzunhisarcikli M, Kalender S, Durak D, Bayrakdar F, Kalender Y. The effects of organophosphate insecticide diazinon on malondialdehyde levels and myocardial cells in rat heart tissue and protective role of vitamin E. Pestic Biochem Physiol 2006; 86: 93-8e.

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Year: 2020
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DOI: 10.2174/2212796814999200818094328
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