Title:Investigation of the Effect of Loratadine and Calcium Ions on Oxidoreductase Activity of Catalase Enzyme
VOLUME: 17 ISSUE: 1
Author(s):Edhem Hasković, Safija Herenda*, Zehra Halilović, Snežana Unčanin, Denis Hasković and Ena Deljkić
Affiliation:Department of Biology, Faculty of Science, University of Sarajevo, Zmaja od Bosne 33-35, 71000 Sarajevo, Department of Chemistry, Faculty of Science, University of Sarajevo, Zmaja od Bosne 33-35, 71000 Sarajevo, Department of Biology, Faculty of Science, University of Sarajevo, Zmaja od Bosne 33-35, 71000 Sarajevo, Clinical Center, University of Sarajevo, Clinic of Nephrology, Bolnička 25, 71 000 Sarajevo, Clinical Center, University of Sarajevo, Organizational Unit Clinical Pathology, Cytology and Human Genetics, Bolnicka 25, 71000 Sarajevo, Clinical Center, University of Sarajevo, Organizational Unit Clinical Biochemistry with Immunology, Bolnička 25, 71 000 Sarajevo
Keywords:Calcium ions, catalase, enzyme inhibition, inhibitor, loratadine, spectrophotometric method.
Abstract:
Background: The Spectrophotometric method is one of the most suitable analytical techniques
for testing the activity of enzymes under the influence of various factors.
Methods: The effect of H1-antihistamines of loratadine and calcium ions on enzyme catalase under
in vitro conditions was investigated in this paper.
Results and Discussion: It has been shown that loratadine is a partial inhibitor of catalase, but this
effect is diminished in the presence of calcium ions. Calcium as well as other cations are important
for many biological and cellular functions. The kidneys play a central role in the homeostasis of these
ions. The activity of the catalase enzyme under the given conditions, the type of inhibition, and the
kinetic parameters of the enzyme reaction were determined.
Conclusion: We concluded that loratadine is a partially competitive inhibitor.
