Background: Betulinic Acid (BA) is a lipophilic compound with proven beneficial results in
topical inflammation. Nanogels (NG) are carriers of bioactive compounds with properties that make
them good candidates to treat skin diseases.
Objective: The objective of this study was to evaluate the anti-inflammatory activity of BA carried in
Methods: NG were composed of a nanoemulsion and a crosslinking agent (Carbopol 940®) applied at
three concentrations (0.5, 1, and 1.5 %) and three activation times (6, 12 and 24 h). In order to select the
optimal formulation, the NG were characterized mechanically and micro-structurally followed by
evaluation of the BA anti-inflammatory activity in an in vivo model of auricular edema. We determined
the edema inhibition activity as percent weight. Additionally, the anti-inflammatory activity of NG was
validated through histological analysis.
Results: The formulation with the best viscoelastic properties was the one prepared with 0.5% carbopol
and 6 h of activation. Microstructural examination of this formulation showed mostly spherical structures
with a mean diameter of 65 nm. From the evaluation of edema and the histological analyses, we
established that the NG of BA produced 52% inhibition. In contrast, a conventional gel and free BA
produced 28% and 19% inhibition, respectively.
Conclusion: The NG of BA were found to be good vehicles to treat skin inflammation.