Background: Cinnamomum cassia (C. cassia) is an evergreen tree in China and Southern
and Eastern Asia. In traditional medicine, cinnamon is widely used due to its many bioactivity
Objective: The present novel study aims to evaluate and make a comparison of antioxidant and antiproliferative
activities of different extractions of C. cassia bark using seven solvents having different
polarities. Solvents polarity gradients start with the solvent of lower polarity, n-hexane, and
end with water as the highest polar solvent. Among the extracts, acetone extract contains the highest
phenolic and flavonoid contents; therefore, it is assessed for the ability to protect DNA from
Methods: The extracts are evaluated for total phenolic, flavonoid contents and antioxidant activities,
using FRAP, DPPH, superoxide, and hydroxyl and nitric oxide radicals scavenging assays.
DNA damage protecting activity of the acetone extract is studied with the comet assay. Each of the
extracts is studied for its antiproliferative effect against, MCF-7, MDA-MB-231(breast cancer), and
HT29 (colon cancer), using MTT assay.
Results: The acetone extract exhibited the highest FRAP value, phenolic and flavonoids contents
when compared to the other extracts and could protect 45% mouse fibroblast cell line (3T3-L1)
from DNA damage at 30 μg/ml. The lowest IC50 value in DPPH, superoxide, and hydroxyl radicals
scavenging was noticed in the ethyl acetate extract. IC50 value obtained for the hexane extract was
the lowest compared to the other extracts in scavenging nitric oxide radicals. The hexane extract
showed the highest antiproliferative effect against cancer cells followed by the chloroform extract.
The ethyl acetate extract inhibited the proliferation of only MCF-7 by IC50 of 100 μg/ml, while the
other extracts exhibited no IC50 in all the cancer cells.
Conclusion: C. cassia showed promising antioxidant and anticancer activities with significant
DNA damage protecting effect.