Background: Melanoma causes more than 80% of deaths from all dermatologic cancers. Hence,
screening and identifying effective compounds to inhibit the growth of melanoma have crucial importance in
basic and clinical treatment.
Methods: High throughput screening was performed to screen and identify compounds that have anti-melanoma
ability. Melanoma cell and mouse allograft models were used to examine the anti-tumor effects of Nuciferine
(NCFR). Western blot, qPCR, and lentivirus overexpression were applied to detect the activation of the
TLR4/NF-κB signaling pathway.
Results: NCFR administration significantly suppressed melanoma cell growth and tumor size by inhibiting the
phosphorylation of p65. NCFR treatment also could suppress TNF-α-induced activation of NF-κB signaling.
The anti-tumor effect of NCFR might be mediated by targeting Toll-like receptors 4.
Conclusion: NCFR inhibits melanoma cell growth and suppresses tumor size, which provides potential therapeutic
strategies for melanoma treatment.