Background: Cell adhesion as dynamic interactions between cell-cell and cell-matrix, has an essential role in
cancer cell migration. Integrins as cell membrane receptors are involved in cell adhesion and signal transduction. Aberrant
expression of integrins is associated with the cancer cell adhesion.
Objective: Targeting the process of cell adhesion and migration could be helpful to prevent cancer cell metastasis.
Amygdalin is a cyanoglycoside compound with anti-cancer properties, while its effect on cancer cell adhesion is not
Methods: The cytotoxic effect of amygdalin on breast cancer cell lines (MCF-7 and MDA-MB-231) and human skin
fibroblast cell line as a normal cell, was evaluated through MTT assay. The cell adhesion assay and wound healing assay
were performed to determine amygdalin effects on adhesion and migration of cancer cells. Further analysis was carried
out to evaluate integrin α and β levels through real-time PCR.
Results: We demonstrated that amygdalin diminished the cell viability of both cell lines in a time and dose-dependent
manner, while amygdalin did not have any toxicity on human skin fibroblast cell line in same dosages. Following
amygdalin treatment, the adhesion of both studied cell lines to fibronectin and collagen I decrease, and this reduction is
significantly greater in the case of binding to fibronectin compared to binding to collagen. The MDA-MB-231 cell
migration was decreased greater than MCF-7 cells. The levels of α and β integrin were differentially regulated by
amygdalin in both cancer cell lines.
Conclusion: These results suggest that depending on cancer cell lines, amygdalin affects cancer cell adhesion and