Background: Glioma is the most common tumor of the central nervous system. Hericium erinaceus,
which has been reported to have a variety of pharmacological activities, is a widely used Traditional Chinese
Medicine (TCM), and also a kind of delicious food accepted by the public.
Methods and Results: In this study, two new natural products, compounds 1 and 2, were isolated and identified
from Hericium erinaceus. They were named erinacerin O and erinacerin P, respectively, after the structural
identification, and their effects on human glioma cell line U87 were evaluated. Erinacerin P (2) exhibited obvious
cytotoxicity on human glioma cell line U87. The IC50 value of 2 was 19.32μg/mL. The results showed that
the apoptosis of U87 cells treated with 2 increased and the morphology of U87 cells altered significantly. Flow
cytometry experiment showed that 2 could significantly increase the apoptosis rate of U87 cells and reduce
DNA replication. Western blot results suggested the Bax/capase-3 pathway was involved in the U87 cell apoptosis
induced by 2.
Conclusion: Erinacerin O and Erinacerin P are novel compounds obtained from Hericium erinaceus and Erinacerin
P could be a potential novel glioma inhibitor.