Background: Deoxypodophyllotoxin, isolated from the Traditional Chinese Medicine Anthriscus
sylvestris, is well-known because of its significant anti-tumor activity with strong toxicity in vitro and in vivo.
Objective: In this article, a series of deoxypodophyllotoxin derivatives were synthesized and their anti-tumor
effectiveness was evaluated.
Methods: The anti-tumor activity of deoxypodophyllotoxin derivatives was investigated by the MTT assay
method. Apoptosis percentage was measured by flow cytometer analysis using Annexin-V-FITC.
Results: The derivatives revealed obvious cytotoxicity in the MTT assay by decreasing the number of late
cancer cells. The decrease of Bcl-2/Bax could be observed in MCF-7, HepG2, HT-29, and MG-63 using
Annexin V-FITC. The ratio of Bcl-2/Bax in the administration group was decreased, which was determined
by the ELISA kit.
Conclusion: The derivatives of deoxypodophyllotoxin could induce apoptosis in tumor cell lines by influencing