Derivatives of Deoxypodophyllotoxin Induce Apoptosis through Bcl-2/Bax Proteins Expression

Author(s): Ya-Nan Li, Ni Ning, Lei Song, Yun Geng, Jun-Ting Fan, Chao-Ying Ma*, He-Zhong Jiang*

Journal Name: Anti-Cancer Agents in Medicinal Chemistry
(Formerly Current Medicinal Chemistry - Anti-Cancer Agents)

Volume 21 , Issue 5 , 2021


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Abstract:

Background: Deoxypodophyllotoxin, isolated from the Traditional Chinese Medicine Anthriscus sylvestris, is well-known because of its significant anti-tumor activity with strong toxicity in vitro and in vivo.

Objective: In this article, a series of deoxypodophyllotoxin derivatives were synthesized and their anti-tumor effectiveness was evaluated.

Methods: The anti-tumor activity of deoxypodophyllotoxin derivatives was investigated by the MTT assay method. Apoptosis percentage was measured by flow cytometer analysis using Annexin-V-FITC.

Results: The derivatives revealed obvious cytotoxicity in the MTT assay by decreasing the number of late cancer cells. The decrease of Bcl-2/Bax could be observed in MCF-7, HepG2, HT-29, and MG-63 using Annexin V-FITC. The ratio of Bcl-2/Bax in the administration group was decreased, which was determined by the ELISA kit.

Conclusion: The derivatives of deoxypodophyllotoxin could induce apoptosis in tumor cell lines by influencing Bcl-2/Bax.

Keywords: Deoxypodophyllotoxin derivatives, tumor cell, flow cytometer, Bcl-2/Bax, Anthriscus sylvestris, anti-tumor activity.

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Article Details

VOLUME: 21
ISSUE: 5
Year: 2021
Published on: 30 July, 2020
Page: [611 - 620]
Pages: 10
DOI: 10.2174/1871520620999200730160952
Price: $65

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