Background: Alfuzosin is recently co-formulated with solifenacin for relieving two coincident
urological diseases, namely; benign prostate hyperplasia and overactive bladder.
Objective: Herein, green, simple and rapid spectrophotometric methods were firstly developed for simultaneous
determination of the two cited drugs in their co-formulated pharmaceutical capsule.
Methods: Alfuzosin, which is the major component in the dosage form, was directly assayed at its extended
wavelength at 330.0 nm. The challenging spectrum of the minor component, solifenacin, was
resolved by five spectrophotometric methods, namely; Dual Wavelength (DW) at 210.0 & 230.0 nm,
first derivative (1D) at 222.0 nm, Ratio Difference (RD) at 217.0 - 271.0 nm , derivative ratio (1DD) at
223.0 and mean centering of ratio spectra (MC) at 217.0 nm.
Results: The proposed methods were successfully validated as per ICH guidelines. Alfuzosin showed
linearity over the range of 4.0 - 70.0 μg/mL, while that of solifenacin were 4.0 - 50.0 μg/mL for DW,
2.0 - 70.0 μg/mL for 1D and RD methods, 1.0 - 70.0 μg/mL for 1DD and 4.0 - 70.0 μg/mL for MC
method. Statistical comparison with their official ones showed no noticeable differences. The methods
showed good applicability for assaying drugs in their newly combination. Besides the eco-scale, the
greenness profile of the methods was assessed and compared with the reported spectrophotometric one
via the newest metric tool; Green Analytical Procedure Index (GAPI).
Conclusion: The proposed methods are superior in not only being smart, accurate, selective, robust and
time-saving, but also in using distilled water as an eco-friendly and cheap solvent.