Introduction: This study introduces an effective strategy, which combines high performance
liquid chromatography coupled with diode array detection (HPLC-DAD) with multivariate calibration
methods for the simultaneous determination of paracetamol (PAR), pseudoephedrine HCl (PSE), dextromethorphan
HBr (DEX) and doxylamine succinate (DOX) along with sweetener saccharin (SAC) in
Methods: PLS-2 and PCR calibration algorithms were selected for data processing. Based on the strategy,
all target analytes were rapidly quantified within 5.3 min under the simple isocratic elution (water:
methanol, 20/80, v/v) without a complete separation. The performances of the proposed methods were
confirmed by analyzing a series of synthetic solutions including different concentrations of analytes.
Results: The average recovery values were in the range of 100.33 to 103.70%, and the REP (relative
error of prediction) values ranged from 1.96 to 4.36% showed that these methods could provide satisfactory
Conclusion: Novel HPLC methods coupled with PLS and PCR algorithm enable a simple, fast and
low-cost analysis of similar pharmaceutical products for simultaneous determination of the target compounds.