Voltammetric Analysis of Ephedrine in Pharmaceutical Dosage Forms and Urine Using poly(Nile Blue A) Modified Glassy Carbon Electrode

Author(s): Fatma Ağın, Gökçe Öztürk, Dilek Kul*

Journal Name: Combinatorial Chemistry & High Throughput Screening
Accelerated Technologies for Biotechnology, Bioassays, Medicinal Chemistry and Natural Products Research

Volume 24 , Issue 3 , 2021

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Objective: The electrochemical analysis of ephedrine which is a sympathometric drug has been studied using poly(Nile blue A) modified glassy carbon electrodes, cyclic voltammetry, differential pulse voltammetry and square wave voltammetry.

Methods: The modified electrodes were prepared by potential cycling electropolymerization of Nile blue A in 0.1 M phosphate buffer solution at pH 6.0. The redox behavior of ephedrine was investigated in different buffer solutions at pH values between 5.5 and 9.0.

Results: Scan rate studies showed that the electron transfer reaction of ephedrine was diffusion controlled. A linear response was obtained between the peak current and the ephedrine concentration in the range of 0.6 to 100 μM with a limit of detection of 2.91×10-3 μM for differential pulse voltammetry in Britton-Robinson buffer solution at pH 9.0. The linearity range of ephedrine in human urine was between 1.0 and 100 μM with a detection limit of 8.16 nM.

Conclusion: The recovery studies in both pharmaceutical dosage forms and urine showed that the proposed method ensured good selectivity, precision and accuracy without any interference from inactive excipients.

Keywords: Ephedrine, poly(Nile blue A), voltammetry, modified glassy carbon electrode, electropolymerization, pharmaceuticals.

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Article Details

Year: 2021
Published on: 18 February, 2021
Page: [366 - 375]
Pages: 10
DOI: 10.2174/1386207323666200727100231
Price: $65

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