Gallic Acid Improved Amytryptiline Effect in Neuropathic Pain Induced by Partial Sciatic Nerve Ligation (PSNL) in Rats

(E-pub Ahead of Print)

Author(s): Prasad Neerati*, Vinay Enumula

Journal Name: The Natural Products Journal

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Aim and Backround: Amytriptiline (AMY) a antidepressant used in the treatment of neuropathic pain. However with limited therapeutic benefits and side effects of AMY is not helpful to majority of the neuropathic pain patients. Gallic acid (GA) is a polyphenolic product with potential antioxidant effects and useful in neuropathic pain.

Objective: The purpose of the study was to investigate the improved neuropathic pain relief with GA in combination with AMY in rats.

Methods: Partial sciatic nerve ligation (PSNL) method is used for neuropathic pain induction in rats. The rats were randomly divided into 6 groups (n = 8), and treated with drugs or vehicle once daily for 15 days after the pain induction. The first group was served as Normal control and received normal saline (p.o), second group was served as Sham Control and received Normal Saline (p.o), third group served as Surgery Control, received normal saline (p.o), fourth group was received with AMY (25mg/kg, p.o), fifth group was received with GA (100mg/kg,p.o), sixth group was received with both AMY and GA. Further behavioural studies for pain were carried out on days 1,4,7,10,13,16,19 and 22. Histological studies of sciatic nerves carried out on days 1, 15 and 22 and on 22nd day all the remaining animals were sacrificed for sciatic nerve tissues biochemical estimations.

Results: GA attenuates neuropathic pain better in combination with AMY by ameliorating the behavioral, biochemical and histopathological signs and symptoms. This effect resulting due to its antioxidant, antinociceptive and anti-inflammatory actions. It also potentiates AMY antinociceptive and antioxidant effects when given in combination in a continuous treatment.

Conclusion: The results suggest that GA in combination with AMYattenuated the neuropathic pain. Hence, the use of GA as an adjunctive with AMY in the neuropathic pain will be more therapeutically beneficial and further studies in patients to confirm the results is warranted.

Keywords: Amytriptiline, Gallic acid, Neuropathic pain, Partial sciatic nerve ligation (PSNL), behavioral studies, sciatic nerve tissues.

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(E-pub Ahead of Print)
DOI: 10.2174/2210315510999200723162912
Price: $95

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