A New 1,2-Benzisoxazolin-3-one Synthetized from Eugenol Shows Anti- Candida Spp. Activity, Specially Against Opportunistic Candida Glabrata

Author(s): Antonio M. Fregnan, Antonio A. Fernandes Júnior, Dalila J. Alvarenga, Josidel C. Oliver, Naiara C. Silva, Luiz F.L. Coelho, Amanda L. Tranches Dias, Danielle F. Dias, Thiago B. de Souza, Diogo T. Carvalho*

Journal Name: Current Bioactive Compounds

Volume 17 , Issue 5 , 2021


Article ID: e010621184056
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Abstract:

Background: In view of the need of discovering new antifungal agents, the synthesis of compounds comprising new structural scaffolds is an important approach, since they may act by new mechanisms of action and overcome resistance issues.

Objective: Synthesis of new 1,2-benzisoxazolin-3-ones inspired in eugenol and ebselen as new antifungal agents against Candida spp.

Methods: A series of 1,2-benzisoxazolin-3-ones, based on eugenol and analogs, was synthesized by the cyclization of the respective hydroxamic acids under Mitsunobu conditions. IR, NMR, and MS spectroscopies characterized the synthesized compounds. The final products were screened for their antifungal activity against five Candida spp., using microdilution assays and cytotoxicity against peripheral blood mononuclear cells by the MTT method.

Results: The products were obtained in good yields and the results showed that most of the synthesized compounds were better antifungal agents than eugenol against the Candida species evaluated and that the derivative IVd (IC50 53 μmol L-1) showed activity comparable to fluconazole against C. glabrata. Moreover, IVd showed the best selectivity profile among the synthesized new compounds.

Conclusion: A new 1,2-benzisoxazolin-3-one (IVd) was obtained as a promising candidate for the development of new antifungal agents based on a new chemical scaffold.

Keywords: 1, 2-benzisoxazolin-3-ones, eugenol, molecular hybridization, Candida spp., antifungals, MTT method.

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Article Details

VOLUME: 17
ISSUE: 5
Year: 2021
Published on: 23 July, 2020
Article ID: e010621184056
Pages: 8
DOI: 10.2174/1573407216999200723114241
Price: $65

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