Background: Currently, ovulation stimulation with clomiphene citrate is the most commonly
used treatment for infertility. It stimulates the secretion of gonadotropins necessary for the release of
Objective: In this study, microextraction method of liquid phase was utilized using hollow fiber with
high-performance liquid chromatography and ultraviolet (HPLC-UV) detector for pre-concentration
and measuring clomiphene citrate in plasma.
Method: Clomiphene citrate was extracted from a 15 ml sample solution with pH=11 in an organic
solvent (Octanol), which was located in the vent wall of the fiber. Subsequently, the drug was introduced
into an internal organic solvent in an acidic pH=2 receptor that was located inside the fiber. In
the following, effective factors in microextraction including the pH of the donor phases and acceptor
phases, the type of organic solvent, ion phase strength, extraction time and stirring speed were investigated
Results: After extracting the drug with an optimal pre-concentration factor of 212, extraction recovery
was 91%, the detection limit was 1.50 ng/ml, limiting the linearity of 5-1000 ng/mL and %RSD=6.98
Conclusion: The proposed method was successfully implemented and optimized for trace detection of
clomiphene citrate in plasma, therefore the clinical and investigational application of this method is
facilitated by this study.