Background: The genus Acer contains around 200 species, with more than 400 garden varieties.
There is considerable diversity in these species and garden varieties, and each can be characterized by morphology
and chemical composition. The red appearance of Acer leaves is due to anthocyanin compounds, including
cyanidin glycosides, delphinidin glycosides, and galloylated anthocyanins. Few studies have investigated the
diversity of anthocyanin compounds in garden varieties, and no studies have examined the pharmacological
effects of these compounds.
Objective: The purpose of this study was to identify the anthocyanins of Acer palmatum cv. ‘Chishio’, a garden
variety of A. palmatum and evaluate their antiproliferative and antioxidant activities.
Methods: A methanol extract of fresh leaves was partitioned with ethyl acetate. The extract was purified by
column chromatography and compounds were subsequently identified by 1H and 13C NMR and ESI-HRMS.
Antiproliferative activity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-
sulfophenyl)-2H-tetrazolium, inner salt (MTS) colorimetric assay. The antioxidant assay was evaluated by scavenging
activity using the stable radical DPPH.
Results: The anthocyanins cyanidin-3-O-(6′′-O-α-rhamnopyranosyl- β-glucopyranoside), cyanidin-3-O- β-
glucopyranoside, cyanidin-3-O-[2′′-O-(galloyl)-6′′-O-(rhamnosyl)-β-glucoside], and cyanidin-3-O-[2′′-O-(galloyl)-
β-glucopyranoside] were isolated from A. palmatum cv. ‘Chishio’. All four anthocyanin compounds showed
antiproliferative activity against LLC and T47D cells, and galloylated anthocyanin showed antiproliferative
activity against C3H10T1/2 cells. All four anthocyanins inhibited the activity of DPPH radicals in a dosedependent
Conclusion: Maple anthocyanins could be a new cancer therapeutic agent or prophylactic medicine.