Aim: The rapid growth, morbidity and mortality of prostate cancer, and the lack of effective treatment
have attracted great interest of researchers to find novel cancer therapies aiming at the effect of gossypin on cell
proliferation and apoptosis of PC-3 cells.
Methods: The effect of gossypin on cell viability was determined using MTT assay at 5-100μg/ml and cisplatin
(50μM) in a time-dependent manner in PC-3 cell lines. The expression levels of caspase-3 (CASP3) and
caspase-9 (CASP9) for apoptosis and Nuclear Factor Kappa B (NFKB1) for survival, inflammation, and growth
were evaluated by real-time PCR. Hoechst staining was used to analyze apoptosis.
Results: Gossypin showed an anti-proliferative effect on PC3 cell line in a time- and dose-dependent manner. In
addition, gossypin led to a significant increase in apoptosis genes (CASP3, CASP9) when compared to control
while it caused a decrease in the level of NFKB1, which is accepted as apoptosis inhibitor (p<0.05) (cisplatin-like).
Gossypin 50 and 100μM significantly induced apoptotic mechanism in PC-3 cells. However, no apoptotic or
commonly stained nuclei have been observed in control group cells.
Conclusion: The results indicated that gossypin can be defined as a promising anticancer agent for PC-3 human
prostate cancer cell line.