With the development of single-cell mRNA sequencing (scRNA-seq), researchers have attempted
to identify new methods for performing in-depth studies of immune cells. However, the discrepancies
between the mRNA levels and the levels of surface proteins have confused many researchers.
Here, we report a significant and interesting phenomenon in which the mRNA and protein expression
levels were mismatched in immune cells. We concluded that scRNA-seq should be combined
with other sequencing methods in single-cell studies (e.g., CITE-seq). The simultaneous assessment of
both mRNA and protein expression will enhance the precision and credibility of the results.
Keywords: Single-cell mRNA sequencing, immune cell, mRNA, protein, myeloid-derived cells, protein expression.
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