Title:Discrepant mRNA and Protein Expression in Immune Cells
VOLUME: 21 ISSUE: 8
Author(s):Jiawei Li, Yi Zhang, Cheng Yang* and Ruiming Rong*
Affiliation:Shanghai Key Laboratory of Organ Transplantation, Shanghai, Shanghai Key Laboratory of Organ Transplantation, Shanghai, Shanghai Key Laboratory of Organ Transplantation, Shanghai, Shanghai Key Laboratory of Organ Transplantation, Shanghai
Keywords:Single-cell mRNA sequencing, immune cell, mRNA, protein, myeloid-derived cells, protein expression.
Abstract:With the development of single-cell mRNA sequencing (scRNA-seq), researchers have attempted
to identify new methods for performing in-depth studies of immune cells. However, the discrepancies
between the mRNA levels and the levels of surface proteins have confused many researchers.
Here, we report a significant and interesting phenomenon in which the mRNA and protein expression
levels were mismatched in immune cells. We concluded that scRNA-seq should be combined
with other sequencing methods in single-cell studies (e.g., CITE-seq). The simultaneous assessment of
both mRNA and protein expression will enhance the precision and credibility of the results.
