Background: Resistance to antimicrobial agents is a major public health problem, being
Staphylococcus aureus prevalent in infections in hospital and community environments and, admittedly,
related to biofilm formation in biotic and abiotic surfaces. Biofilms form a complex and structured
community of microorganisms surrounded by an extracellular matrix adhering to each other and to a
surface that gives them even more protection from and resistance against the action of antimicrobial
agents, as well as against host defenses.
Methods: Aiming to control and solve these problems, our study sought to evaluate the action of 1,2,3-
triazoles against a Staphylococcus aureus isolate in planktonic and in the biofilm form, evaluating the
activity of this triazole through Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal
Concentration (MBC) tests. We have also performed cytotoxic evaluation and Scanning Electron Microscopy
(SEM) of the biofilms under the treatment of the compound. The 1,2,3-triazole DAN 49
showed bacteriostatic and bactericidal activity (MIC and MBC 128 μg/mL). In addition, its presence
interfered with the biofilm formation stage (1/2 MIC, p <0.000001) and demonstrated an effect on
young preformed biofilm (2 MICs, p <0.05).
Results: Scanning Electron Microscopy images showed a reduction in the cell population and the appearance
of deformations on the surface of some bacteria in the biofilm under treatment with the compound.
Conclusion: Therefore, it was possible to conclude the promising anti-biofilm potential of 1,2,3-triazole,
demonstrating the importance of the synthesis of new compounds with biological activity.