Background: Metabolomics has gained importance in clinical applications over the last
decade. Metabolomics studies are significant because the systemic metabolome is directly affected
by disease conditions. Metabolome-based biomarkers are actively being developed for early diagnosis
and to indicate the stage of specific diseases. Additionally, understanding the effect of an intervention
on a living organism at the molecular level is a crucial strategy for understanding novel or
unexpected biological processes.
Results: The simultaneous improvements in advanced analytical techniques, sample preparation
techniques, computer technology, and databank contents have enabled more valuable scientific information
to be gained from metabolomics than ever before. With over 15,000 known endogenous
metabolites, there is no single analytical technique capable of analyzing the whole metabolome.
However, capillary electrophoresis-mass spectrometry (CE-MS) is a unique technique used to analyze
an important portion of metabolites not accessible by liquid chromatography or gas chromatography
techniques. The analytical capability of CE, combined with recent sample preparation techniques
focused on extracting polar-ionic compounds, makes CE-MS a perfect technique for metabolomic
Conclusion: Here, previous reviews of CE-MS based metabolomics are evaluated to highlight recent
improvements in this technique. Specifically, we review papers from the last two years (2018 and
2019) on CE-MS based metabolomics. The current situation and the challenges faced by metabolomic
studies are discussed to reveal the high potential of CE-MS.