Preparation of β-CD–Quercetin Complex and its Effects on Ethanol- Damaged BRL-3A Hepatocytes

Author(s): Yingxia Zhang, Xiao Lin, Jinglong Wang, Sun Jing, Deya Wang, Zhongjing Tian, Meiling Kang, Chengshi Ding*, Shishui He*, Jing Ma*

Journal Name: Current Drug Delivery

Volume 17 , Issue 8 , 2020

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Abstract:

Objective: To prepare the sustained-release complex, quercetin was incorporated with βcyclodextrin (β-CD) and the effect of β-CD–quercetin complex on the growth of ethanol-injuried hepatocytes was studied.

Methods: By using scanning electron microscopy, infrared spectroscopy, and release rate analysis, βCD–quercetin complex was identified. The effect of different concentrations of β-CD–quercetin complex on the growth of ethanol-damaged hepatocytes at different time was observed by using MTT assay, and the cell quantity and morphology were observed by using hematoxylin–eosin staining. By using single-cell gel electrophoresis, the prevention of β-CD–quercetin complex from the DNA damage of ethanol-damaged BRL-3A cells was studied, and Olive tail moment was calculated.

Results: β-CD–quercetin complex as the sustained-release complex was successfully prepared. The ethanol induced damage of BRL-3A cells could be prevented by 20, 40 and 80 mg/L of quercetin complex, and the protection mechanism of hepatocyte was related to the antioxidation of DNA.

Conclusion: Quercetin sustained-release complex could be prepared with β-CD, and it might be used to treat alcoholic liver disease.

Keywords: β-CD–quercetin complex, preparation, ethanol-induced hepatocyte injury, prevention of the DNA damage, antioxidation, spectroscopy.

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Article Details

VOLUME: 17
ISSUE: 8
Year: 2020
Page: [720 - 726]
Pages: 7
DOI: 10.2174/1567201817666200708114738
Price: $95

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