Background: Osteoarthritis (OA), one of the most important causes leading to joint
disability, was considered as an untreatable disease. A series of genes were reported to regulate the
pathogenesis of OA, including microRNAs, Long non-coding RNAs and Circular RNA. So far, the
expression profiles and functions of lncRNAs, mRNAs, and circRNAs in OA are not fully
Objective: The present study aimed to identify differentially expressed genes in OA.
Methods: The present study conducted RNA-seq to identify differentially expressed genes in OA.
Ontology (GO) analysis was used to analyze the Molecular Function and Biological Process. KEGG
pathway analysis was used to perform the differentially expressed lncRNAs in biological pathways.
Results: Hierarchical clustering revealed a total of 943 mRNAs, 518 lncRNAs, and 300 circRNAs,
which were dysregulated in OA compared to normal samples. Furthermore, we constructed
differentially expressed mRNAs mediated protein-protein interaction network, differentially
expressed lncRNAs mediated trans-regulatory networks, and competitive endogenous RNA
(ceRNA) to reveal the interaction among these genes in OA. Bioinformatics analysis revealed that
these dysregulated genes were involved in regulating multiple biological processes, such as wound
healing, negative regulation of ossification, sister chromatid cohesion, positive regulation of
interleukin-1 alpha production, sodium ion transmembrane transport, positive regulation of cell
migration, and negative regulation of inflammatory response. To the best of our knowledge, this
study for the first time, revealed the expression pattern of mRNAs, lncRNAs and circRNAs in OA.
Conclusion: This study provided novel information to validate these differentially expressed RNAs
may be as possible biomarkers and targets in OA.