Variation and heterogeneity between cells are the basic characteristics of stem cells. Traditional
sequencing analysis methods often cover up this difference. Single-cell sequencing technology
refers to the technology of high-throughput sequencing analysis of genomes at the single-cell level. It
can effectively analyze cell heterogeneity and identify a small number of cell populations. With the
continuous progress of cell sorting, nucleic acid extraction and other technologies, single-cell sequencing
technology has also made great progress. Encouraging new discoveries have been made in stem
cell research, including pluripotent stem cells, tissue-specific stem cells and cancer stem cells. In this
review, we discuss the latest progress and future prospects of single-cell sequencing technology in the
field of stem cells.
Keywords: Single-cell sequencing, pluripotent stem cell, tissue-specific stem cell, cancer stem cell, RNA sequencing, heterogeneity.
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