Analysis of Orphenadrine Citrate in Various Chiral Stationary Phases: A Comparative Study

Author(s): Prachi Raikar*, Bannimath M. Gurupadayya, Sripuram Subramanyam, Gunnam Srinivasu

Journal Name: Current Chromatography

Volume 7 , Issue 2 , 2020

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Abstract:

Background: Polysaccharide based chiral stationary phases (CSPs) were used to perform enantiomeric separation of Orphenadrine Citrate by Ultra-Fast Liquid Chromatography (UFLC) technique. Trials were conducted using the polar mode, reverse phase mode and normal phase mode. Amylose and Cellulose-based CSPs were used for the same.

Materials and Methods: Eight Amylose-based CSPs and four Cellulose-based CSPs were used in the reverse phase mode. Five Amylose-based CSPs and two Cellulose-based CSPs were used in polar mode. The only Cellulose-based CSP used in the normal phase mode could effectively separate Orphenadrine Citrate enantiomers with a good resolution.

Results and Discussion: Successful enantioseparation was obtained using Chiralcel OD-H containing Cellulose tris (3, 5- dimethylphenylcarbamate) as a chiral selector and n-hexane: Ethanol: Diethylamine (95: 05: 0.1, v/v/v) as the mobile phase. The developed method was validated in accordance with ICH guidelines (Q2R1).

Conclusion: The proposed objectives were successfully accomplished as the developed method could effectively resolve Orphenadrine Citrate enantiomers.

Keywords: Chiral stationary phase, enantioseparation, orphenadrine citrate, ultra-fast liquid chromatography, amylose-based CSPs, cellulose-based CSPs.

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Article Details

VOLUME: 7
ISSUE: 2
Year: 2020
Published on: 29 December, 2020
Page: [91 - 100]
Pages: 10
DOI: 10.2174/2213240607999200621202159
Price: $25

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