Background: Polysaccharide based chiral stationary phases (CSPs) were used to perform
enantiomeric separation of Orphenadrine Citrate by Ultra-Fast Liquid Chromatography (UFLC)
technique. Trials were conducted using the polar mode, reverse phase mode and normal phase mode.
Amylose and Cellulose-based CSPs were used for the same.
Materials and Methods: Eight Amylose-based CSPs and four Cellulose-based CSPs were used in
the reverse phase mode. Five Amylose-based CSPs and two Cellulose-based CSPs were used in polar
mode. The only Cellulose-based CSP used in the normal phase mode could effectively separate
Orphenadrine Citrate enantiomers with a good resolution.
Results and Discussion: Successful enantioseparation was obtained using Chiralcel OD-H containing
Cellulose tris (3, 5- dimethylphenylcarbamate) as a chiral selector and n-hexane: Ethanol: Diethylamine
(95: 05: 0.1, v/v/v) as the mobile phase. The developed method was validated in accordance
with ICH guidelines (Q2R1).
Conclusion: The proposed objectives were successfully accomplished as the developed method
could effectively resolve Orphenadrine Citrate enantiomers.
Keywords: Chiral stationary phase, enantioseparation, orphenadrine citrate, ultra-fast liquid chromatography, amylose-based
CSPs, cellulose-based CSPs.
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