Background: Salinomycin, an ionophore antibiotic, has a strong anti-cancer effect, inducing
the apoptosis of cancer cells and cancer stem cells.
Objective: The aim of the study was to assess the influence of salinomycin on the expression profile of
genes related to stemness and miRNA regulating their expression in endometrial cancer cells.
Methods: Endometrial cancer cells of cell line Ishikawa were exposed to salinomycin at concentrations
in the range of 0.1-100 μM, with the aim of determining its pro-apoptotic potential and the concentration
which would cause the death of 50% of the cells (Sulforhodamine B test). In the following stages,
the cells were incubated with the drug at a concentration of 1μM for 12,24 and 48 hour periods and
compared to the control. Determining the changes in the expression of the genes related to stemness
and regulating their miRNA was done using the microarray technique and RTqPCR. ELISA assay was
performed in order to determine the level of TGFβ2, COL14A1, CDH2, WNT5A in cell culture under
salinomycin treatment in comparison to the control.
Results: Salinomycin caused the apoptosis of cells. For the concentration of 0.1 μM, a decrease in the
population of living cells by 11.9% was determined. For 1 μM, it was 49.8%, for 10 μM -69.4%, and
for a concentration of 100 μM - 87.9%. The most noticeable changes in the expression caused by the
addition of salinomycin into the culture were noted for mRNA: TGFβ2; WNT5A (up-regulated);
COL14A1; CDH2 (down-regulated), as well as miRNA: hsa-miR-411 (up-regulated); hsa-miR-200a;
hsa-miR-33a; hsa-miR-199a; hsa-miR-371-5p; hsa-miR-374; hsa-miR-374b (down-regulated).
Conclusion: It was confirmed that salinomycin has an influence on the stemness process. The most noticeable
changes in the expression were noted for mRNA: TGFβ2; COL14A1; CDH2; WNT5A, as well
as for miRNA: hsa-miR-200a; hsa-miR-33a; hsa-miR-199a; hsa-miR-371-5p; hsa-miR-411; hsa-miR-