Background: The World Health Organization added snakebite envenoming to its priority
list of neglected tropical diseases. Snakebite envenoming is a particularly important public
health problem in rural areas of tropical and sub-tropical countries. In 2017, more than 30 thousand
cases of snakebites were reported in Brazil, with the North and Northeast regions being the most
affected and Bothrops sp snakes causing most of these cases.
Objective: The objective of this study was to evaluate, in vitro, the coagulating, cytotoxic, oxidizing
and antioxidant effects caused by Bothrops jararacussu and B. moojeni crude venoms.
Methods: The crude venoms protein profiles were characterized, and the biological effects were
evaluated and compared between the species.
Results: The crude venoms composition showed similar protein profiles. The B. jararacussu and B.
moojeni crude venoms showed coagulant activity and were capable of causing indirect hemolysis
on the erythrocyte membrane, but did not protect the erythrocyte membrane from damage against
hypotonic solutions. The B. jararacussu crude venom promoted hemagglutination.
Conclusion: The crude venoms were not able to promote an oxidizing effect on hemoglobin and
could not prevent the phenylhydrazine oxidizing effect; however, the crude venoms presence
caused increase in the methemoglobin formation by phenylhydrazine oxidation.