Title:Using Alendronic Acid Coupled Fluorescently Labelled SM Liposomes as a Vehicle for Bone Targeting
VOLUME: 26 ISSUE: 46
Author(s):Oula P. Medina*, Tuula P. Medina*, Jana Humbert, Bao Qi, Wolfgang Baum, Olga Will, Timo Damm and Claus Glüer
Affiliation:Section Biomedical Imaging, Department of Radiology and Neuroradiology, University Medical Center Schleswig-Holstein (UKSH), Kiel University, Kiel, Section Biomedical Imaging, Department of Radiology and Neuroradiology, University Medical Center Schleswig-Holstein (UKSH), Kiel University, Kiel, Section Biomedical Imaging, Department of Radiology and Neuroradiology, University Medical Center Schleswig-Holstein (UKSH), Kiel University, Kiel, Section Biomedical Imaging, Department of Radiology and Neuroradiology, University Medical Center Schleswig-Holstein (UKSH), Kiel University, Kiel, Universitatsklinikum Erlangen, Medizinische Klinik 3 Institut für Klinische Immunologie, Gluckstrasse 4A, 91054 Erlangen, Section Biomedical Imaging, Department of Radiology and Neuroradiology, University Medical Center Schleswig-Holstein (UKSH), Kiel University, Kiel, Section Biomedical Imaging, Department of Radiology and Neuroradiology, University Medical Center Schleswig-Holstein (UKSH), Kiel University, Kiel, Section Biomedical Imaging, Department of Radiology and Neuroradiology, University Medical Center Schleswig-Holstein (UKSH), Kiel University, Kiel
Keywords:Alendronic acid, liposomes, multimodal imaging, ICG, sphingomyelin, bone targeting.
Abstract:Background: We recently developed a liposomal nanoparticle system that can be used for drug delivery
and simultaneously be monitored by optical or photoacoustic imaging devices. Here we tested the efficacy of
alendronate as a homing molecule in SM-liposomes for bone targeting.
Methods: Alendronate was immobilized covalently on the liposomal surface and the fluorescent dye indocyanine
green was used as a payload in the liposomes. The indocyanine green delivery was analyzed by 3D optical tomography,
optical fluorescence scanner, photoacoustic imaging, and by ex-vivo biodistribution studies.
Results: The results show that the alendronate, coupled to the liposomal surface, increases sphingomyelin containing
liposome targeting up to several-folds.
Conclusion: The alendronate targeted liposomes open possibilities for an application in active bone targeting.
