Background: Brucellosis is an economically important zoonotic disease caused by the gram negative bacteria
belonging to the genus Brucella. Medicinal plants are well known for a wide variety of potential antimicrobial agents that
can be used as anti-microbial drugs.
Method: In the present study, crude ethanol and methanol extracts of local plants (Berberies lyceum and Fagonia cretica)
were tested in vitro against Brucella melitensis via well diffusion method for their antibacterial activity. In in-silico study,
phytochemicals previously identified in the selected plants were docked with homology model of the cytotoxic factor malate synthase G (MSG) highly conserved among Brucella spp., in Molecular Operating Environment (MOE) to predict a potential drug against B. melitensis. Molecular dynamic simulation was performed to predict the stability of MSG through
Result: Ethanolic crude extracts of B. lyceum showed maximum zone of inhibition (32.5 mm) followed by methanolic extracts (30 mm), while ethanolic extracts of F. cretica showed zone of inhibition (29 mm) followed by methanolic extracts
(27.5 mm). In silico screening predicted phytic acid as the most potent inhibitor followed by jehlumine, barbamine, oxyberberine and sindamine.
Conclusion: The synergistic utilization of phytochemicals derived from B. lyceum may potentially provide protection
against B. melitensis.