Background: Previous studies reported the inevitable destructive effects of radiotherapy on normal
adjacent cells. Ascorbic Acid (AA) has been proposed as an effective anti-cancer agent with no obvious effects
on normal cells.
Objective: The effects of Ascorbic acid in combination with radiotherapy on human pancreatic carcinoma cell
line were studied.
Methods: The human pancreatic cancer cells were cultured and divided into four groups: control group (A)
without any treatment, group B that received 2Gy radiotherapy alone, group C that was treated with 4mM AA
alone, and group D that was co-treated with AA and radiotherapy. Cell viability, DNA fragmentation, expression
of apoptotic genes, and Reactive Oxygen Species (ROS) production were determined in treated cells.
Results: There was a noticeable decrease in cell viability after treatment with AA (and/or) radiotherapy. All
treated groups showed elevated ROS production, Bax/Bcl2 expression, DNA fragmentation, and cytotoxycity
compared with the control group. Cells under combination therapy showed the most cytotoxicity.
Conclusion: The results suggest that AA at a dose of 4mmol/l may be used as an effective radio-sensitizing
agent in pancreatic cancer cell line.