Background: In Algerian traditional medicine, Centaurea species are well known in
traditherapy. Centaurea africana has been used in folk medicine for the treatment of several inflammatory
Objective: This study aims to examine the antioxidant, anti-inflammatory and anti-proliferative potential
of both n-Butanol (BECA) and ethyl acetate (EAECA) extracts of Centaurea africana.
Methods: The phytochemical analysis of both BECA and EAECA were explored and the antioxidant
activities were investigated by measuring the DPPH° scavenging effect, the reducing power
and the inhibition of lipid peroxidation (LPO) induced by by Fe2+/ ascorbic acid system. The antiinflammatory
properties were determined by measuring the NO° scavenging effect and by using
carrageenan-induced rat paw oedema. The antiproliferative activity was studied on HT29 (human
colorectal adenocarcinoma), OV2008 (human ovarian cancer) and C6 (Rattus norvegicus brain glioma)
cell lines using the Sulforhodamine B assay.
Results: The total polyphenol contents (TPC) of EAECA and BECA are recorded at 125.24±10.14
and 53.03±2.50 mgGAE/g extract, respectively. Both extracts revealed the antioxidant activity in a
concentration-dependent manner; this effect is more pronounced with EAECA. The BECA exhibited
a higher anti-inflammatory activity. This anti-inflammatory activity was reflected in a reduction
of swelling of carrageenan-evoked edemas (48.45 %), inhibition of nitric oxide (84.7 %), effective
decrease in myeloperoxidase activity (58.82 %) and malondialdehyde level (65.58 %). The cytotoxic
effect of BECA was found to be more pronounced against C6 cell lines (IC50 value: 131.93
μg/mL) while the cytotoxic activity of EAECA was more effective against HT29 and OV2008 cell
Conclusion: The obtained results indicated that EAECA exhibited a high antioxidant activity,
while BECA has significant anti-inflammatory activity. Both extracts showed cytotoxic effects
against cancer cell lines at certain concentrations in a cell-specific manner.