Title:Antioxidant, Anti-Inflammatory and Cytotoxic Properties of <i>Centaurea africana</i> Lamk var. [Bonnet] M
VOLUME: 20 ISSUE: 1
Author(s):Sabrina Hadjira, Amira Mansour, Caglar Berkel, Ramdane Seghiri, Ahmed Menad, Fadila Benayache, Samir Benayache, Ercan Cacan and Souad Ameddah*
Affiliation:Laboratoire de Biologie et Environnement, Faculte des Sciences de la Nature et de la Vie, Universite des Freres Mentouri Constantine 1, Constantine, Unite de Valorisation des Ressources Naturelles, Molecules Bioactives et Analyse Physicochimiques et Biologiques (VARENBIOMOL), Universite desFreresMentouri Constantine 1, Constantine, Department of Molecular Biology and Genetics, Tokat Gaziosmanpasa University, 60250 Tokat, Unite de Valorisation des Ressources Naturelles, Molecules Bioactives et Analyse Physicochimiques et Biologiques (VARENBIOMOL), Universite desFreresMentouri Constantine 1, Constantine, Laboratoire de Biologie et Environnement, Faculte des Sciences de la Nature et de la Vie, Universite des Freres Mentouri Constantine 1, Constantine, Unite de Valorisation des Ressources Naturelles, Molecules Bioactives et Analyse Physicochimiques et Biologiques (VARENBIOMOL), Universite desFreresMentouri Constantine 1, Constantine, Unite de Valorisation des Ressources Naturelles, Molecules Bioactives et Analyse Physicochimiques et Biologiques (VARENBIOMOL), Universite desFreresMentouri Constantine 1, Constantine, Department of Molecular Biology and Genetics, Tokat Gaziosmanpasa University, 60250 Tokat, Laboratoire de Biologie et Environnement, Faculte des Sciences de la Nature et de la Vie, Universite des Freres Mentouri Constantine 1, Constantine
Keywords:Centaurea africana, phenolic content, antioxidant activity, anti-inflammatory activity, antiproliferative activity,
flavonoids, oxidative stress.
Abstract:
Background: In Algerian traditional medicine, Centaurea species are well known in
traditherapy. Centaurea africana has been used in folk medicine for the treatment of several inflammatory
disorders.
Objective: This study aims to examine the antioxidant, anti-inflammatory and anti-proliferative potential
of both n-Butanol (BECA) and ethyl acetate (EAECA) extracts of Centaurea africana.
Methods: The phytochemical analysis of both BECA and EAECA were explored and the antioxidant
activities were investigated by measuring the DPPH° scavenging effect, the reducing power
and the inhibition of lipid peroxidation (LPO) induced by by Fe2+/ ascorbic acid system. The antiinflammatory
properties were determined by measuring the NO° scavenging effect and by using
carrageenan-induced rat paw oedema. The antiproliferative activity was studied on HT29 (human
colorectal adenocarcinoma), OV2008 (human ovarian cancer) and C6 (Rattus norvegicus brain glioma)
cell lines using the Sulforhodamine B assay.
Results: The total polyphenol contents (TPC) of EAECA and BECA are recorded at 125.24±10.14
and 53.03±2.50 mgGAE/g extract, respectively. Both extracts revealed the antioxidant activity in a
concentration-dependent manner; this effect is more pronounced with EAECA. The BECA exhibited
a higher anti-inflammatory activity. This anti-inflammatory activity was reflected in a reduction
of swelling of carrageenan-evoked edemas (48.45 %), inhibition of nitric oxide (84.7 %), effective
decrease in myeloperoxidase activity (58.82 %) and malondialdehyde level (65.58 %). The cytotoxic
effect of BECA was found to be more pronounced against C6 cell lines (IC50 value: 131.93
μg/mL) while the cytotoxic activity of EAECA was more effective against HT29 and OV2008 cell
lines.
Conclusion: The obtained results indicated that EAECA exhibited a high antioxidant activity,
while BECA has significant anti-inflammatory activity. Both extracts showed cytotoxic effects
against cancer cell lines at certain concentrations in a cell-specific manner.
