Aim and Objective: Oxidative stress is implicated in the development and progression
of many diseases. Some of the appropriate actions that could taken to resolve the problem of these
diseases are search for new antioxidant substances isolated from plants. The aims of this study
were to research the intraspecies variations of A. verticillata and C. caeruleus essential oils from 8
locations using statistical analysis, the in vitro antioxidant properties of collective essential oils and
Materials and Methods: The essential oils were analyzed by GC and GC-MS. The intraspecies
variations of the essential oil compositions were discussed using principal component analysis
(PCA) and cluster analysis (CA). The antioxidant properties were evaluated DPPH-radical
scavenging activity and β-carotene bleaching test.
Results: The main components of Ammoides verticillata collective essential oil (Coll EO) were
thymol (30.5%), carvacrol (23.2%), p-cymene (13.1%), limonene (12.5%) and terpinene-4-ol
(12.3%). While roots of Carthamus caeruleus essential oil were dominated by carline oxide
(86.2%). The chemical variability allowed the discrimination of two main Groups for both Coll
EOs. A direct correlation between the altitudes, climate and the chemical compositions was
evidenced. Ammoides verticulata and Carthamus caeruleus Coll Eos showed good antioxidant
activity. In binary mixture, the interaction between both Coll Eos and between oils rich of thymol
and/or carvacrol with carlina oxide produced the best synergistic effects compared to individual
essential oils and the synthetic antioxidant (BHT).
Conclusion: Ammoides verticillata and Carthamus caeruleus essential oil blends can be used as a
natural food preservative and alternative to chemical antioxidants.