Background: The cardiac system is a combination of a complex structure, various cells,
and versatile specified functions and sophisticated regulatory mechanisms. Moreover, cardiac diseases
that encompass a wide range of endogenous conditions, remain a serious health burden worldwide.
Recent genome-wide profiling techniques have taken the lead in uncovering a new realm of cell types
and molecular programs driving physiological and pathological processes in various organs and diseases.
In particular, the emerging technique single-cell RNA sequencing dominates a breakthrough in
decoding the cell heterogeneity, phenotype transition, and developmental dynamics in cardiovascular
Conclusion: Herein, we review recent advances in single cellular studies of cardiovascular system and
summarize new insights provided by single-cell RNA sequencing in heart developmental sciences,
stem-cell researches as well as normal or disease-related working mechanisms.