Background: Many topical agents are available in the market, which interfere with the pigmentation
process at different levels. They are often known to cause side effects ranging from irritation
to tumor over chronic use.
Objective: The present study was designed to develop and characterize an anti blemish cream containing
Methods: A herbal cream was formulated using dill, nagarmotha and black cumin oil and subjected to
evaluation of its anti blemish potential against stress augmented UV-B rays-induced hyperpigmentation.
Topical oil in water type of creams containing 2%, 4% and 6% of each oil was formulated using
herbal oils. The formulated cream was characterized for solubility, pH, particle size, grittiness, viscosity,
stability, phase separation, shelf life and spreadability, and found to be stable. Acute dermal toxicity
was carried out individually for dill, nagarmotha and black cumin oil according to the OECD guidelines
402. Hyperpigmentation was induced in all the experimental animals by stress-augmented UV-B
irradiation method. The animals were treated for 30 days (twice daily) with standard and test formulations
by topical administration, whereas the disease group was left untreated. The skin of the animals
was subjected to photographical study as well as grading for pigmentation and irritation before and
after treatment. After the treatment period, the serum antioxidant levels were estimated and histopathology,
histochemical studies of skin were performed.
Results: The animals treated with test formulations containing 2%, 4%, and 6% of herbal oil showed
significant improvement in pigmentation compared to disease control as it is evident in photographic
biochemical, histopathological and histochemical studies.
Conclusion: Thus, it was concluded that the developed anti-blemish cream containing herbal oils possesses
significant anti-blemish potential. This study necessitates further evaluations in human subjects
as it could have a high positive therapeutic value in the treatment of hyperpigmentation.