Development, Characterization and Pharmacological Evaluation of Antiblemish Cream Containing Herbal Oils

Author(s): Sathiya Krishnaraj*, Komala Mahadevappa, Radhika Muniyappa Narayanaswamy, Devanand Kamnoore, Ramya Lingutla, Tanmoy Ghosh

Journal Name: Recent Patents on Drug Delivery & Formulation
Continued as Recent Advances in Drug Delivery and Formulation

Volume 14 , Issue 3 , 2020


Graphical Abstract:


Abstract:

Background: Many topical agents are available in the market, which interfere with the pigmentation process at different levels. They are often known to cause side effects ranging from irritation to tumor over chronic use.

Objective: The present study was designed to develop and characterize an anti blemish cream containing herbal oils.

Methods: A herbal cream was formulated using dill, nagarmotha and black cumin oil and subjected to evaluation of its anti blemish potential against stress augmented UV-B rays-induced hyperpigmentation. Topical oil in water type of creams containing 2%, 4% and 6% of each oil was formulated using herbal oils. The formulated cream was characterized for solubility, pH, particle size, grittiness, viscosity, stability, phase separation, shelf life and spreadability, and found to be stable. Acute dermal toxicity was carried out individually for dill, nagarmotha and black cumin oil according to the OECD guidelines 402. Hyperpigmentation was induced in all the experimental animals by stress-augmented UV-B irradiation method. The animals were treated for 30 days (twice daily) with standard and test formulations by topical administration, whereas the disease group was left untreated. The skin of the animals was subjected to photographical study as well as grading for pigmentation and irritation before and after treatment. After the treatment period, the serum antioxidant levels were estimated and histopathology, histochemical studies of skin were performed.

Results: The animals treated with test formulations containing 2%, 4%, and 6% of herbal oil showed significant improvement in pigmentation compared to disease control as it is evident in photographic biochemical, histopathological and histochemical studies.

Conclusion: Thus, it was concluded that the developed anti-blemish cream containing herbal oils possesses significant anti-blemish potential. This study necessitates further evaluations in human subjects as it could have a high positive therapeutic value in the treatment of hyperpigmentation.

Keywords: Antiblemish cream, herbal oil, hyperpigmentation, UV-B irradiation.

[1]
Brenner M, Hearing VJ. The protective role of melanin against UV damage in human skin. Photochem Photobiol 2008; 84(3): 539-49.
[http://dx.doi.org/10.1111/j.1751-1097.2007.00226.x] [PMID: 18435612]
[2]
Hancock A. Note on the excavating Sponges; with descriptions of four new species. J Nat Hist 1867; 19(112): 229-42.
[3]
Serup J, Keiding J, Fullerton A, Gniadecka M, Gniadecki R. Highfrequency ultrasound examination of skin: Introduction and guide 2006; 2: 473-91.
[http://dx.doi.org/10.3109/9781420003307-68]
[4]
Ortonne JP, Bissett DL. Latest insights into skin hyperpigmentation. J Investig Dermatol Symp Proc 2008; 13(1): 10-4.
[http://dx.doi.org/10.1038/jidsymp.2008.7] [PMID: 18369333]
[5]
D’Mello SA, Finlay GJ, Baguley BC, Askarian-Amiri ME. Signaling pathways in melanogenesis. Int J Mol Sci 2016; 17(7): 1144.
[http://dx.doi.org/10.3390/ijms17071144] [PMID: 27428965]
[6]
Videira IF, Moura DF, Magina S. Mechanisms regulating melanogenesis. An Bras Dermatol 2013; 88(1): 76-83.
[http://dx.doi.org/10.1590/S0365-05962013000100009] [PMID: 23539007]
[7]
Yoon JH, Shim JS, Cho Y, et al. Depigmentation of melanocytes by isopanduratin A and 4-hydroxypanduratin A isolated from Kaempferia pandurata ROXB. Biol Pharm Bull 2007; 30(11): 2141-5.
[http://dx.doi.org/10.1248/bpb.30.2141] [PMID: 17978489]
[8]
Stulberg DL, Clark N, Tovey D. Common hyperpigmentation disorders in adults: Part I. Diagnostic approach, café au lait macules, diffuse hyperpigmentation, sun exposure, and phototoxic reactions. Am Fam Physician 2003; 68(10): 1955-60.
[PMID: 14655804]
[9]
Yamaguchi Y, Iribe G, Nishida M, Naruse K. Role of TRPC3 and TRPC6 channels in the myocardial response to stretch: Linking physiology and pathophysiology. Prog Biophys Mol Biol 2017; 130: 264-72.
[http://dx.doi.org/10.1016/j.pbiomolbio.2017.06.010] [PMID: 28645743]
[10]
Chahal KK, Monika AK, Bhardwaj U, Kaur R. Chemistry and biological activities of Anethum graveolens L (dill) essential oil: A review. Journal of Pharmacognosy and Phytochemical 2017; 6(2): 295-306.
[11]
Radulescu V, Popescu ML, Ilies DC. Chemical composition of the volatile oil from different plant parts of Anethum graveolens L. Farmacia 2010; 58(5): 594-600.
[12]
Srivastava RK, Singh A, Srivastava CP, et al. Chemical constituents and biological activities of promising aromatic plant nagarmotha (Cyperus scariosus R. Br.): A review In Proc Indian Natn Sci Acad 2014; 80(3): 525-36.
[13]
Alobaidi AH. Hamad, Kudair KA, Alsamarai AM. Formulation of hypopigmentation cream and evaluation of its effect on skin pigment, Part I: Formulof the Product. Dermatol 2014; 5(1): 9.
[http://dx.doi.org/10.7241/ourd.20141.02]
[14]
Eid AM, Elmarzugi NA, Abu Ayyash LM, Sawafta MN, Daana HI. A review on the cosmeceutical and external applications of Nigella sativa. J Trop Med 2017.7092514
[http://dx.doi.org/10.1155/2017/7092514] [PMID: 29358959]
[15]
Taddeo VA, Epifano F, Preziuso F, Fiorito S, Caron N, Rives A. hplc analysis and skin whitening effects of umbelliprenin-containing extracts of Anethum graveolens, Pimpinella anisum, and Ferulago campestris. Mol 2019; 24(3): 501.
[16]
Naseri M, Mojab F, Khodadoost M, et al. The study of anti-inflammatory activity of oil-based dill (Anethum graveolens L.) extract used topically in formalin-induced inflammation male rat paw. Iran J Pharm Res 2012; 11(4): 1169-74.
[PMID: 24250550]
[17]
Ha RBK, Osborne R, Szczepaniak RJ. Procter and Gamble Co Skincare composition comprising dill extract US Patent Application 12/691 2010; 471.
[18]
Shyu YS, Lin JT, Chang YT, Chiang CJ, Yang DJ. Evaluation of antioxidant ability of ethanolic extract from dill (Anethum graveolens L.) flower. Food Chem 2009; 15115(2): 515-21.
[19]
Aljabre SH, Alakloby OM, Randhawa MA. Dermatological effects of Nigella sativa. JDDS 2015; 19(2): 92-8.
[http://dx.doi.org/10.1016/j.jdds.2015.04.002]
[20]
Inomata S, Kobayashi K. Shiseido Co Ltd External skin preparations for suppressing sebum secretion US Patent Application 11/270 2006; 438.
[21]
Burits M, Bucar F. Antioxidant activity of Nigella sativa essential oil. Phytother Res 2000; 14(5): 323-8.
[http://dx.doi.org/10.1002/1099-1573(200008) ] [PMID: 10925395]
[22]
Jungersted JM, Hellgren LI, Jemec GB, Agner T. Lipids and skin barrier function--a clinical perspective. Contact Dermat 2008; 58(5): 255-62.
[http://dx.doi.org/10.1111/j.1600-0536.2008.01320.x] [PMID: 18416754]
[23]
Purnamawati S, Indrastuti N, Danarti R, Saefudin T. The role of moisturizers in addressing various kinds of dermatitis: A review. Clin Med Res 2017; 15(3-4): 75-87.
[http://dx.doi.org/10.3121/cmr.2017.1363] [PMID: 29229630]
[24]
Gharby S, Harhar H, Guillaume D, et al. Chemical investigation of Nigella sativa L. seed oil produced in Morocco. J Saudi Soc Agric Sci 2015; 14(2): 172-7.
[http://dx.doi.org/10.1016/j.jssas.2013.12.001]
[25]
Aswal A, Kalra M, Rout A. Preparation and evaluation of poly herbal cosmetic cream. Der Pharmacia Lettre 2013; 5(1): 83-8.
[26]
Saad AH, Ahmed SN, Mohamed EB. Formulation and evaluation of herbal cream from Ziziphusspina leaves extract. Int Res J Pharm 2013; 4: 44-8.
[27]
Saraf S, Jeswani G, Kaur CD, Saraf S. Development of novel herbal cosmetic cream with Curcuma longa extract loaded transfersomes for antiwrinkle effect. Afr J Pharm Pharmacol 2011; 5(8): 1054-62.
[28]
Dhase AS, Khadbadi SS, Saboo SS. Formulation and evaluation of vanishing herbal cream of crude drugs. Am J Ethnomed 2014; 1: 313-8.
[29]
Auluck A, Rosin MP, Zhang L, Sumanth KN. Oral submucous fibrosis, a clinically benign but potentially malignant disease: Report of 3 cases and review of the literature. J Can Dent Assoc 2008; 74(8): 735-40.
[PMID: 18845065]
[30]
Moysan A, Clément-Lacroix P, Michel L, Dubertret L, Morlière P. Effects of ultraviolet A and antioxidant defense in cultured fibroblasts and keratinocytes. Photodermatol Photoimmunol Photomed 1996; 11(5-6): 192-7.
[http://dx.doi.org/10.1111/j.1600-0781.1995.tb00168.x] [PMID: 8738713]
[31]
Gurcan MN, Boucheron LE, Can A, Madabhushi A, Rajpoot NM, Yener B. Histopathological image analysis: a review. IEEE Rev Biomed Eng 2009; 2(2): 147-71.
[http://dx.doi.org/10.1109/RBME.2009.2034865] [PMID: 20671804]
[32]
Arellano JM, Storch V, Sarasquete C. Ultrastructural and histochemical study on gills and skin of the senegal sole, Soleasenegalensis. J Appl Ichthyology 2004; 20(6): 452-60.
[http://dx.doi.org/10.1111/j.1439-0426.2004.00543.x]
[33]
Nair X, Tramposch KM. UVB-induced pigmentation in hairless mice as an in vivo assay for topical skin-depigmenting activity. Skin Pharmacol 1989; 2(4): 187-97.
[http://dx.doi.org/10.1159/000210819] [PMID: 2638867]
[34]
Ramu S, Mahendra SA, Kunchakuri K, Karadi PP, Swetha K, Radhakrishnan G. Isolation, characterisation and in vitro screening of anticataract potential of Fucoidan from Sargassum wightii Greville. J Herbmed Pharmacol 2018; 7(4)
[http://dx.doi.org/10.15171/jhp.2018.37]
[35]
Finlay AY. Quality of life measurement in dermatology: A practical guide. Br J Dermatol 1997; 136(3): 305-14.
[http://dx.doi.org/10.1111/j.1365-2133.1997.tb14934.x] [PMID: 9115906]
[36]
Halliwell B. Biochemistry of oxidative stress. Biochem Soc Trans 2007; 35(5): 1147-50.
[http://dx.doi.org/10.1042/BST0351147] [PMID: 17956298]
[37]
Sies H. Biochemistry of oxidative stress. Angew Chem Int Ed Engl 1986; 25(12): 1058-71.
[http://dx.doi.org/10.1002/anie.198610581]
[38]
Sies H. Oxidative stress: oxidants and antioxidants. Exp Physiol 1997; 82(2): 291-5.
[http://dx.doi.org/10.1113/expphysiol.1997.sp004024] [PMID: 9129943]
[39]
Morliere P, Moysan A, Tirache I. Action spectrum for UV-induced lipid peroxidation in cultured human skin fibroblasts. Free Radic Biol Med 1995; 19(3): 365-71.
[http://dx.doi.org/10.1016/0891-5849(95)00043-W] [PMID: 7557551]
[40]
Rukmini MS, D’Souza B, D’Souza V. Superoxide dismutase and catalase activities and their correlation with malondialdehyde in schizophrenic patients. Indian J Clin Biochem 2004; 19(2): 114-8.
[http://dx.doi.org/10.1007/BF02894268] [PMID: 23105467]
[41]
Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 1967; 70(1): 158-69.
[PMID: 6066618]
[42]
Costin GE, Hearing VJ. Human skin pigmentation: melanocytes modulate skin color in response to stress. FASEB J 2007; 21(4): 976-94.
[http://dx.doi.org/10.1096/fj.06-6649rev] [PMID: 17242160]
[43]
Lin CH, Ding HY, Kuo SY, Chin LW, Wu JY, Chang TS. Evaluation of in vitro and in vivo depigmenting activity of raspberry ketone from Rheum officinale. Int J Mol Sci 2011; 12(8): 4819-35.
[http://dx.doi.org/10.3390/ijms12084819] [PMID: 21954327]
[44]
Hachiya A, Sriwiriyanont P, Kasamatsu S. Kao corp animal model for hyperpigmentation. US 7973211, 2011.
[45]
Forrest IS, Kosek JC, Aber RC, Serra MT. Rabbit as a model for chlorpromazine-induced hyperpigmentation of the skin. Biochem Pharmacol 1970; 19(3): 849-52.
[http://dx.doi.org/10.1016/0006-2952(70)90246-7] [PMID: 5507689]
[46]
Inoue K, Hosoi J, Ideta R, Ohta N, Ifuku O, Tsuchiya T. Stress augmented ultraviolet-irradiation-induced pigmentation. J Invest Dermatol 2003; 121(1): 165-71.
[http://dx.doi.org/10.1046/j.1523-1747.2003.12326.x] [PMID: 12839577]
[47]
Matsuda H, Nakamura S, Kubo M. Studies of cuticle drugs from natural sources. II. Inhibitory effects of Prunus plants on melanin biosynthesis. Biol Pharm Bull 1994; 17(10): 1417-20.
[http://dx.doi.org/10.1248/bpb.17.1417] [PMID: 7874069]
[48]
Tabassum N, Hamdani M. Plants used to treat skin diseases. Pharmacogn Rev 2014; 8(15): 52-60.
[http://dx.doi.org/10.4103/0973-7847.125531] [PMID: 24600196]
[49]
Aswal A, Kalra M, Rout A. Preparation and evaluation of polyherbal cosmetic cream. Der Pharmacia Lettre 2013; 5(1): 83-8.
[50]
Sklar LR, Almutawa F, Lim HW, Hamzavi I. Effects of ultraviolet radiation, visible light, and infrared radiation on erythema and pigmentation: a review. Photochem Photobiol Sci 2013; 12(1): 54-64.
[http://dx.doi.org/10.1039/C2PP25152C] [PMID: 23111621]
[51]
Svobodová AR, Galandáková A, Sianská J, Doležal D, Ulrichová J, Vostálová J. Acute exposure to solar simulated ultraviolet radiation affects oxidative stress-related biomarkers in skin, liver and blood of hairless mice. Biol Pharm Bull 2011; 34(4): 471-9.
[http://dx.doi.org/10.1248/bpb.34.471] [PMID: 21467631]


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VOLUME: 14
ISSUE: 3
Year: 2020
Page: [223 - 232]
Pages: 10
DOI: 10.2174/1872211314666200601163458
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