Aim and Objective: Cells and tissues of the body are prone to oxidative damage as a result of
increased level of reactive oxygen species and nitrogen radical beyond the detoxifying ability of the
endogenous antioxidant system. This study aimed to evaluate the ameliorative effect of methanolic
extracts of Nigella sativa (MENS) against cadmium-induced blood oxidative stress and testicular
toxicity in albino rats.
Materials and Methods: Twenty five (25) male albino rats, weighing (200±20g), were randomly
grouped into five groups (A-E). Group B (Negative Control) received intraperitoneal administration
of cadmium chloride (CdCl2, 5mg/kg) only, group C received CdCl2 and low dose MENS (300mg/kg,
oral), group D received CdCl2 and high dose MENS (600mg/kg, oral), group E (Positive control)
received CdCl2 and Vitamin C (200mg/kg, oral), for 14 days. No treatment was administered to group
A (Normal control). The oxidative state of the blood was assessed by measuring the blood levels or
activities of MDA, CAT, GSH and SOD; while testicular injury was assessed by measuring serum
testosterone level using ELISA. The testes were harvested for histopathological examination.
Results: The results showed that cadmium induced a marked elevation in the level of MDA, and a
decrease in SOD, CAT and GSH levels or activities (p<0.05 or p<0.01); but no significant alteration
in the serum testosterone level (p>0.05); Histopathological studies on the testes showed that cadmium
significantly induced testicular injury, which was however ameliorated by the seed extract of
Conclusion: We conclude that N.sativa seed extract is potentially testiculoprotective and attenuates
oxidative stress against harmful chemical toxins such as cadmium.