Background: The development of innovative approaches in drug analysis is a challenging
task for medicine, pharmacy, and engineering sciences. For instance, the requirement of proper dosage
forms in releasing active ingredients is crucial. It is essential to analyze drugs in biological liquids
for early diagnosis and treatment purposes. Drug analysis is also of great importance to control
the quality of pharmaceutical products, test their efficacy, and develop novel drug formulation. The
present review is aimed to highlight the most recent spectrophotometric approaches applied to analyze
various classes of drugs in biological media and/or dosage forms.
Methods: Direct and derivative UV/UV-Vis spectrophotometry and a combination of various techniques
with spectrophotometry, such as injection analysis and chemometrics, have most widely been
applied in the analysis of dosage forms. In addition, emerging technologies, such as UV imaging allows
to obtain the distribution of drug concentration in time-resolved 2D images based on UV light
absorption, utilization of nanotechnology, self-assembled nanomaterials, and aptamer-based nanoparticles
have gained interests to investigate drug assays and to quantify the proper drug release.
Results: Due to their high versatility, ease of application, low cost, and fast response, spectrophotometric
methods are one of the most preferable methods providing high accuracy and precision with a
wide linear range in drug analysis.
Conclusion: Selected examples demonstrating the applicability of spectrophotometric methods in
pharmaceutical assays in this review might contribute to the overall importance of the analytical test
used in modern pharmaceutical analysis.