Background: Quinolones are an important group of synthetic antibiotics with bactericidal
action. The antibacterial activity of these compounds has been shown to result from selective inhibition
of bacterial DNA synthesis. It is a preferable option in clinical use because of its efficacy, reliability
and wide antibacterial spectrum. The identification and quantification of quinolones in various
biological tissues and fluids are needed.
Methods: Numerous techniques have been developed for their analysis of biological materials and
pharmaceutical dosage forms. In this review, the chromatographic methods published in the last ten
years for the analysis of quinolones, using for the treatment of an infection in a human, were reviewed.
Results: The analysis methods of quinolones as single or simultaneous in biological materials were
summarized according to sample preparation, chromatographic conditions, validation parameters and
applicability of the methods. The pH effect on the chromatographic behaviors of quinolones was discussed.
Moreover, multivariate analysis was performed for the summarized published methods based
on the method parameters.
Conclusion: Many rapid and efficient separation methods have been developed for individual or
simultaneous determination of quinolone. In this study, a comprehensive review is presented for the
analysis of quinolone developed in the last decade.