Generic placeholder image

Current Chemical Biology

Editor-in-Chief

ISSN (Print): 2212-7968
ISSN (Online): 1872-3136

Research Article

Nigella sativa Seed Protects Against Cadmium-induced Renal Toxicity in Rats

Author(s): Ikenna Kingsley Uchendu* and Henshaw Uchechi Okoroiwu

Volume 14, Issue 2, 2020

Page: [140 - 149] Pages: 10

DOI: 10.2174/2212796814666200520083818

Price: $65

Abstract

Background: Prevalence of chemical-induced renal injuries has been on a fast rise over the years and has become the leading cause of mortality and morbidity in the society, with environmental pollutants, heavy metals inclusive, seen as the causal agents. Recently, the role of medicinal foods in human health has gained considerable attention.

Objective: We investigated the protective effects of methanolic extract of Nigella sativa (MENS) (Black seed) against cadmium-induced renal toxicity in albino rats.

Methods: Twenty-five (25) male albino rats, weighing (150-170g), were randomly grouped into five groups: A-E. Group B (Negative Control) received intraperitoneal administration of cadmium chloride (CdCl2, 5mg/kg) only, group C received CdCl2 and low dose MENS (300mg/kg, oral), group D received CdCl2 and high dose MENS (600mg/kg, oral), group E (Positive control) received CdCl2 and Vitamin C (200mg/kg, oral), for 7 days. No treatment was administered to group A (Normal control). Renal injury was assessed by measuring serum levels of Na+, K+, creatinine and blood urea nitrogen (BUN) using standard methods. The kidneys were harvested for histopathological examination.

Results: CdCl2 induced significant nephrotoxicity with marked elevation in the levels of biochemical markers of renal functions (p<0.05 or p<0.01); these were, however, ameliorated by a low dose of MENS. Histopathological examination of the kidney sections supported the biochemical findings.

Conclusion: We conclude that Nigella sativa seed extract, at a low dose, is potentially nephroprotective against harmful chemical toxins such as cadmium.

Keywords: Cadmium, CdCl2, Nigella sativa, Medicinal food, Nephroprotection, Nutritional biochemistry.

Graphical Abstract
[1]
Ahmad A, Husain A, Mujeeb M, et al. A review on therapeutic potential of Nigella sativa: A miracle herb. Asian Pac J Trop Biomed 2013; 3(5): 337-52.
[http://dx.doi.org/10.1016/S2221-1691(13)60075-1] [PMID: 23646296]
[2]
Hosseini M, Mohammadpour T, Karami R, Rajaei Z, Reza Sadeghnia H, Soukhtanloo M. Effects of the hydro-alcoholic extract of Nigella sativa on scopolamine-induced spatial memory impairment in rats and its possible mechanism. Chin J Integr Med 2015; 21(6): 438-44.
[http://dx.doi.org/10.1007/s11655-014-1742-5] [PMID: 24584756]
[3]
Amin B, Hosseinzadeh H. Black cumin (Nigella sativa) and its active constituent, thymoquinone: An overview on the analgesic and anti-inflammatory effects. Planta Med 2016; 82(1-2): 8-16.
[PMID: 26366755]
[4]
Kellum JA, Prowle JR. Paradigms of acute kidney injury in the intensive care setting. Nat Rev Nephrol 2018; 14(4): 217-30.
[http://dx.doi.org/10.1038/nrneph.2017.184] [PMID: 29355173]
[5]
Workeneh BT. Acute Kidney Injury. J Inj Violence Res 2018; 16(1): 3-7.
[PMID: 29103063]
[6]
Hoste EAJ, Kellum JA, Selby NM, et al. Global epidemiology and outcomes of acute kidney injury. Nat Rev Nephrol 2018; 14(10): 607-25.
[http://dx.doi.org/10.1038/s41581-018-0052-0] [PMID: 30135570]
[7]
Bienholz A, Kribben A, Feldkamp T. Acute kidney injury: New insights from diagnostics and treatment. Dtsch Med Wochenschr 2012; 137(13): 652-5.
[PMID: 22434174]
[8]
Allen JH. The wicked problem of chemicals policy: Opportunities for innovation. J Environ Sci (China) 2013; 3: 101-8.
[9]
Schyman P, Printz RL, Estes SK, Boyd KL, Shiota M, Wallqvist A. Identification of the toxicity pathways associated with thioacetamide-induced injuries in rat liver and kidney. Front Pharmacol 2018; 9: 1272.
[http://dx.doi.org/10.3389/fphar.2018.01272] [PMID: 30459623]
[10]
Shen J, Wang X, Zhou D, et al. Modelling cadmium-induced cardiotoxicity using human pluripotent stem cell-derived cardiomyocytes. J Cell Mol Med 2018; 22(9): 4221-35.
[http://dx.doi.org/10.1111/jcmm.13702] [PMID: 29993192]
[11]
Borné Y, Barregard L, Persson M, Hedblad B, Fagerberg B, Engström G. Cadmium exposure and incidence of heart failure and atrial fibrillation: A population-based prospective cohort study. BMJ Open 2015; 5(6)e007366
[http://dx.doi.org/10.1136/bmjopen-2014-007366] [PMID: 26078311]
[12]
Ajibesin KK. Dacryodes edulis (G. Don) HJ. Lam: A review on its medicinal, phytochemical and economic properties. Res J Med Plant 2011; 5(1): 32-41.
[http://dx.doi.org/10.3923/rjmp.2011.32.41]
[13]
Ekeigwe IB, Ikegwuonu IC, Uchendu IK, Uchenna CA, Okongwu UC. Curcuma longa aqueous extract prevents myocardial injury in hypercholesterolaemic albino rat. Ukr Biochem J 2019; 91(4): 50-7.
[http://dx.doi.org/10.15407/ubj91.04.050]
[14]
Babaei HA, Motamedifar M, Khalifat S, Mohammadi A, Khosrow Z, Motamedifar A. In vitro study of antibacterial property and cytotoxic effects of aqueous, ethanolic, methanolic, and hydroalcoholic extracts of fenugreek seed. Pak J Med Health Sci 2018; 12(2): 906-10.
[15]
Lorke D. A new approach to practical acute toxicity testing. Arch Toxicol 1983; 54(4): 275-87.
[http://dx.doi.org/10.1007/BF01234480] [PMID: 6667118]
[16]
Trease G, Evans SM. Pharmacognosy. 15th ed. Edinburgh, New York: WB Saunders 2002.
[17]
Fogh-Andersen N, Wimberley PD, Thode J, Siggaard-Andersen O. Determination of sodium and potassium with ion-selective electrodes. Clin Chem 1984; 30(3): 433-6.
[http://dx.doi.org/10.1093/clinchem/30.3.433] [PMID: 6697490]
[18]
Natelson S, Scott ML, Beffa C. A rapid method for the estimation of urea in biologic fluids. Am J Clin Pathol 1951; 21(3): 275-81.
[http://dx.doi.org/10.1093/ajcp/21.3_ts.275] [PMID: 14818981]
[19]
Fabiny DL, Ertingshausen G. Automated reaction-rate method for determination of serum creatinine with the CentrifiChem. Clin Chem 1971; 17(8): 696-700.
[http://dx.doi.org/10.1093/clinchem/17.8.696] [PMID: 5562281]
[20]
Baker FJ, Silverton RE. Pallister CJ Baker and Silverton’s introduction to laboratory technology. 7th ed. Wobrun, MA, USA: Butterworth-Heinemann 1998; p. 448.
[21]
Okamoto R, Ali Y, Hashizume R, Suzuki N, Ito M. BNP as a major player in the heart-kidney connection. Int J Mol Sci 2019; 20(14): 3581.
[http://dx.doi.org/10.3390/ijms20143581] [PMID: 31336656]
[22]
Kiyuna LA, Albuquerque RPE, Chen CH, Mochly-Rosen D, Ferreira JCB. Targeting mitochondrial dysfunction and oxidative stress in heart failure: Challenges and opportunities. Free Radic Biol Med 2018; 129: 155-68.
[http://dx.doi.org/10.1016/j.freeradbiomed.2018.09.019] [PMID: 30227272]
[23]
Siva K, Ramnath V, Jeyaprakash K. Effect of Silymarin on cadmium induced toxicity in rats. World J Pharm Res 2018; 7: 1193-205.
[24]
Bücker-Neto L, Paiva ALS, Machado RD, Arenhart RA. Margis- Pinheiro M. Interaction between plant hormones and heavy metals responses. Genet Mol Biol 2017; 40(1): 373-86.
[http://dx.doi.org/10.1590/1678-4685-gmb-2016-0087] [PMID: 28399194]
[25]
Erdem O, Yazihan N, Kocak MK, Sayal A, Akcil E. Influence of chronic cadmium exposure on the tissue distribution of copper and zinc and oxidative stress parameters in rats. Toxicol Ind Health 2016; 32(8): 1505-14.
[http://dx.doi.org/10.1177/0748233714566875] [PMID: 25647810]
[26]
Pradeepkumar B, Sudheer A, Reddy TS, Reddy KS, Narayana G, Veerab-hadrappa K. Cardioprotective activity of flavonoid fraction of gymnema sylvestre leaves on doxorubicin induced cardiac damage. JYP 2018; 10(4): 422-6.
[http://dx.doi.org/10.5530/jyp.2018.10.93]
[27]
Yimer EM, Tuem KB, Karim A, Ur-Rehman K, Anwar F. F. Nigella sativa L (black cumin): A promising natural remedy for wide range of illnesses. Evid- Based Compl Alt Med 2019. 2019. ID 1528635.
[http://dx.doi.org/10.1155/2019/1528635.]
[28]
Dirican A, Atmaca H, Bozkurt E, Erten C, Karaca B, Uslu R. Novel combination of docetaxel and thymoquinone induces synergistic cytotoxicity and apoptosis in DU-145 human prostate cancer cells by modulating PI3K-AKT pathway. Clin Transl Oncol 2015; 17(2): 145-51.
[http://dx.doi.org/10.1007/s12094-014-1206-6] [PMID: 25060568]
[29]
Ismail N, Ismail M, Azmi NH, Abu Bakar MF, Basri H, Abdullah MA. Modulation of hydrogen peroxide-induced oxidative stress in human neuronal cells by thymoquinone-rich fraction and thymoquinone via transcriptomic regulation of antioxidant and apoptotic signaling genes. Oxid Med Cell Longev 2016; 20162528935
[http://dx.doi.org/10.1155/2016/2528935] [PMID: 26823946]
[30]
Paarakh MP. Nigella sativa Linn – A comprehensive review. IJNPR 2010; 1(4): 409-29.
[31]
Akram Khan M, Afzal M. Chemical composition of Nigella sativa Linn: Part 2 Recent advances. Inflammopharmacology 2016; 24(2-3): 67-79.
[http://dx.doi.org/10.1007/s10787-016-0262-7] [PMID: 27068721]
[32]
Saleh FA, El-Darra N, Raafat K, Ghazzawi IE. Phytochemical analysis of Nigella sativa L. Utilizing GC-MS exploring its antimicrobial effects against multidrug-resistant bacteria. Pharmacogn J 2018; 10(1): 99-105.
[http://dx.doi.org/10.5530/pj.2018.1.18]
[33]
Ahmadi M, Scurtu M, Tulcan C, et al. Nigella sativa - a plant with personality in biochemistry and experimental medicine researches. Bulletin UASVM veterinary medicine 2016; 73(2): 203-9.
[34]
Darakhshan S, Bidmeshki Pour A, Hosseinzadeh Colagar A, Sisakhtnezhad S. Thymoquinone and its therapeutic potentials. Pharmacol Res 2015; 95-96: 138-58.
[http://dx.doi.org/10.1016/j.phrs.2015.03.011] [PMID: 25829334]
[35]
Cherrak SA, Mokhtari-Soulimane N, Berroukeche F, et al. In vitro antioxidant versus metal ion chelating properties of flavonoids: A structure-activity investigation. PLoS One 2016; 11(10)e0165575
[http://dx.doi.org/10.1371/journal.pone.0165575] [PMID: 27788249]
[36]
Mahmoud AM, Bautista RJH, Sandhu MA, Hussein OE. Beneficial effects of citrus flavonoids on cardiovascular and metabolic health. Oxid Med Cell Longev 2019.
[http://dx.doi.org/10.1155/2019/5484138]
[37]
Kooti W, Hasanzadeh-Noohi Z, Sharafi-Ahvazi N, Asadi-Samani M, Ashtary-Larky D. Phytochemistry, pharmacology, and therapeutic uses of black seed (Nigella sativa). Chin J Nat Med 2016; 14(10): 732-45.
[http://dx.doi.org/10.1016/S1875-5364(16)30088-7] [PMID: 28236403]
[38]
Panche AN, Diwan AD, Chandra SR. Flavonoids: An overview. J Nutr Sci 2016. 5e47.
[http://dx.doi.org/10.1017/jns.2016.41] [PMID: 28620474]
[39]
Liaudanskas M, Viškelis P, Raudonis R, Kviklys D, Uselis N, Janulis V. Phenolic composition and antioxidant activity of malus domestica leaves. ScientificWorld J 2014; 2014306217
[http://dx.doi.org/10.1155/2014/306217.]
[40]
de Jesus NZT, de Souza Falcão H, Gomes IF, et al. Tannins, peptic ulcers and related mechanisms. Int J Mol Sci 2012; 13(3): 3203-28.
[http://dx.doi.org/10.3390/ijms13033203] [PMID: 22489149]
[41]
Abdel-Daim MM, Ghazy EW. Effects of Nigella sativa oil and ascorbic acid against oxytetracycline-induced hepato-renal toxicity in rabbits. Iran J Basic Med Sci 2015; 18(3): 221-7.
[PMID: 25945233]

Rights & Permissions Print Cite
© 2024 Bentham Science Publishers | Privacy Policy