Background: The existing parenteral treatment of cervical cancer has high toxicity and
poor distribution of drugs at the targeted site.
Purpose: To formulate localized mucoadhesive cisplatin loaded microparticles based formulation
to treat cervical cancer so that enhanced therapeutics benefits with low toxicity could be achieved.
Methods: Cisplatin loaded chitosan coated spray-dried microparticles were prepared by ionotropic
gelation technique and optimized by Central Composite Design. The spray-dried uncoated and chitosan-
coated microparticles were characterized for various parameters (Particle size, Morphology,
Drug entrapment efficiency). In vitro drug release study was carried out in simulated vaginal fluids
by dialysis membrane method. Ex vivo studies were carried out to evaluate the cytotoxic potential
of the developed formulation by the MTT assay. A drug permeability study was performed by
Franz diffusion cell using the vaginal tissue of Swiss Albino Mice.
Results: All in vitro characterization parameters were found to be optimum. The in vitro release
studies indicated a controlled release following the Higuchi model. The chitosan-coated microparticles
were found to be more cytotoxic than uncoated microparticles and plain cisplatin solution. The
chitosan-coated microparticles were found to be more permeable than uncoated microparticles. Finally,
in vivo tumor regression and histopathological studies confirmed the significant decrease in
tumor volume at different time intervals.
Conclusion: Thus, it can be concluded that mucoadhesive spray-dried microparticles could provide
a favorable approach for localized delivery of the anticancer drug via vaginal route against cervical
cancer with its enhanced effectiveness.