Title:A Polyphenolic Complex Attenuates Inflammatory Response and Blood- Brain Barrier Disruption
VOLUME: 17 ISSUE: 3
Author(s):Yuting Bian, Toru Yamashita, Yuki Taira, Jingwei Shang, Keiichiro Tsunoda, Tian Feng, Ryo Sasaki, Xia Liu, Xiaowen Shi, Koh Tadokoro, Emi Nomura, Namiko Matsumoto, Yusuke Osakada, Yoshio Omote, Mami Takemoto, Nozomi Hishikawa, Yasuyuki Ohta and Koji Abe*
Affiliation:Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Department of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama
Keywords:Ischemic stroke, middle cerebral artery occlusion, rosmarinic acid, polyphenolic complex, anti-inflammation, blood
brain barrier.
Abstract:
Background: Cerebral ischemia causes a strong inflammatory response. Neumentix is a
dietary supplement containing 14.9% rosmarinic acid and 29.9% total phenolic content, which has
been proved to be beneficial against inflammatory response. Therefore, Neumentix’s effect on
anti-inflammatory and blood brain barrier (BBB) disruption in transient middle cerebral artery occlusion
(tMCAO) model mice is investigated in this study.
Methods: After the pretreatment of vehicle or Neumentix 134 mg/kg/d, intraperitoneal injection
(i.p.) (containing rosmarinic acid 20 mg/kg/d) for 14 days, mice were subjected to tMCAO for
60 min and kept receiving vehicle or Neumentix daily 5 days afterward.
Results: Neumentix treatment ameliorated neurobehavioral impairment in the corner test (5d after
tMCAO, **P<0.01), reduced infarct volume (#P<0.05), suppressed expression of ionized calciumbinding
adapter molecule-1 (Iba-1), tumor necrosis factor alpha (TNF-α) and monocyte chemoattractant
protein-1 (MCP-1) (###P<0.001), and improved the integrity of BBB (§P<0.05) at 5 days
after tMCAO.
Conclusion: The present study provided an evidence of Neumentix’s anti-inflammatory and neuroprotection
effect against BBB disruption on experimental tMCAO model mice, suggesting that
Neumentix could be a potential therapeutic agent for stroke.