Background: Diabetes is a metabolic disorder affecting a large percentage of the population
worldwide. The chronic hyperglycemic condition leads to the generation of advanced glycation end
products, reactive oxygen species and inflammatory cytokines, which worsen the functioning of the
kidney. Clinical management of diabetic nephropathy is difficult as it requires a multi-focused approach.
Hence, a combination of lisinopril a drug used in clinical practice for nephropathy, and
naringenin, a flavonoid reported to have a significant effect in nephropathy, may show additive or synergistic
effect with less side effects.
Objective: The objective of the present study was to evaluate the effect of a combination of lisinopril
with naringenin in diabetic nephropathy.
Methods: Diabetes was induced in male Sprague Dawley rats by streptozotocin (55 mg/kg, i.p.). After
four weeks of diabetes induction animals were treated with naringenin alone and a combination of
Lisinopril and naringenin for the next four weeks. At the end of the study, various urine and biochemical
parameters were evaluated. Oxidative stress parameters like malondialdehyde, reduced glutathione;
catalase and superoxide dismutase for kidney tissues were estimated and histopathology studies of
kidneys were carried out.
Results: The combination of lisinopril (10 mg/kg) and naringenin (25 and 50 mg/kg) treatment showed
significant improvement in the biochemical and urine parameters. Combination treatment also attenuated
renal oxidative stress and renal damage as observed in histopathological studies.
Conclusion: Treatment with a combination of lisinopril and naringenin showed a promising effect on
diabetic nephropathy in rats.