Background: The therapeutic effects of a number of natural products on
inflammatory bowel disease (IBD) have recently been examined in detail. The whole
herb and roots of Angelica keiskei (Umblliferae) have traditionally been used as a
diuretic, to treat gastrointestinal diseases such as gastric ulcers and diarrhea in Japan.
Objectives: The present study was performed to investigate the effects of xanthoangelol,
a major chalcone of Angelica keiskei roots, on diarrhea and inflammation in the large
intestine of IBD model mice.
Methods: Xanthoangelol (10 & 25 mg/kg) was orally administered to mice with 3%
dextran sulfate sodium (DSS)-induced colitis. Blood samples were collected during the
experimental period, subjected to a full blood count test, and colonic cytokine and
chemokine levels were measured.
Results: Xanthoangelol (25 mg/kg) reduced the disease activity index (DAI) of colitis.
It also attenuated DSS-induced reductions in red blood cell and platelet counts as well
as Hb and Ht levels. A histological examination of the colon using direct fast scarlet
staining showed that xanthoangelol prevented DSS-induced mucosal ulceration and
eosinophil infiltration. Xanthoangelol also reduced DSS-induced increases in colonic
MCP-1, IL-1β and TNF-α levels.
Conclusions: Xanthoangelol reduced DSS-induced increases in colonic IL-1β, TNF-α,
and MCP-1 levels and prevented eosinophil infiltration, which support its potential of as
a treatment for IBD.