Objective: Polydopamine coated iron oxide nanoparticles (Fe3O4@PDA NPs) were synthesized,
characterized, and their MR imaging contrast agents and photothermal potency were evaluated on melanoma
(B16-F10 and A-375) cells and normal skin cells. To this end, MTT assay, Fe concentration, and MR imaging of
both coated and uncoated NPs were assessed in C57BL/6 mice.
Methods: Fe3O4 nanoparticles were synthesized using co-precipitation, and coated with polydopamine. The
cytotoxicity of Fe3O4 and Fe3O4@PDA NPs on melanoma cells, with different concentrations, were obtained
using MTT assay. MR images and Fe concentrations of nanoprobe and nanoparticles were evaluated under in
Results: Findings indicated that uncoated Fe3O4 showed the highest toxicity in animal (B16-F10) cells at
450μg/ml after 72h, while the highest toxicity in human (A-375) cells were observed at 350μg/ml. These
nanoparticles did not reveal any cytotoxicity to normal skin cells, despite having some toxicity features in A-375
cells. MR image signals in the tumor were low compared with other tissues. The iron concentration in the tumor
was higher than that of other organs.
Conclusion: It is concluded that the cytotoxicity of Fe3O4@PDA was found to be significantly lower than uncoated
nanoparticles (p <0.001), which allows some positive effects on reducing toxicity. The prepared nanoprobe
may be used as a contrast agent in MR imaging.