Background: Extensive loss of skin in burn patients can have devastating consequences,
both physically and mentally. Adipose-Derived Stem Cells (ADSCs) and fibroblasts are known to play
significant roles in the process of wound healing. Recently, bioengineered skin has been considered for
wound healing purposes.
Methods: Investigate the effect of Integra seeded with ADSCs, fibroblasts, or both on wound healing.
Results: We found that when Integra is seeded with ADSCs and fibroblasts, both types of cells incorporate
and proliferate, the phenomenon becoming more robust when the cells are co-cultured on Integra,
both in vitro and in vivo. In addition, when these cells are seeded on Integra, they stimulate epithelization
with no signs of inflammation and skin necrosis being observed when transplanted on animals for 7 days.
Conclusion: ADSCs and fibroblasts seeded on Integra could decrease the number of α-SMA positive
myofibroblasts, leading to scarless wound healing. The evidence from this study is strongly supportive
that Integra seeded with ADSCs and fibroblasts is an appropriate and effective bioengineered skin for