Background: Screening of multiple methods is worthless for formulators due to material
losses, wastage of time, and expenditures. It is imperative to make a quick decision.
Objective: The present investigation describes the systematic approach to select the best suitable method
for the development of nanoliposomes (NL), the precursor of nanocochleates encapsulating curcumin
using Analytic Hierarchy Process (AHP).
Methods: Pair-wise comparison matrices were used to achieve the overall priority weight and ranking
for the selection of appropriate technique. Furthermore, Plackett-Burman screening Design (PBD) was
exploited to investigate specific effects of associated formulation and process variables on particle size
(Y1), drug content (Y2), and entrapment efficiency (Y3), while fabricating NL.
Results: Results revealed the reliability of the pair-wise comparison matrices and selected the ethanol
injection method with the highest priority weight (0.337). Bland-Altman plot and control chart validated
the results of AHP. The preparation of vesicles with the preferred diameter and size distribution was
essentially fulfilled. Stirring speed (X5), amount of phospholipid (X4), and cholesterol (X8) showed
significant influence (p<0.05) on Y1 and Y3, PBD revealed. These factors can be further optimized
using the design of experiments.
Conclusion: AHP being an effective tool, has assisted in selecting the best alternative for fabricating
NL, whilst PBD enabled a clear understanding of the effects of diverse formulation variables on
responses studied. Results ensure that NL is a riveting candidate for modulating effectively into tailormade
diverse shaped nanoformulations for further in vitro and in vivo studies.