Giemsa Staining of Some Nano-formulations on Neuroblastoma Cell Line

(E-pub Ahead of Print)

Author(s): Kuldeep Nigam, Shweta Dang*.

Journal Name: Nanoscience & Nanotechnology-Asia

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Background: Nano-formulations of various drugs and natural compounds are becoming popular day by day. Number of cosmetic, medicinal and herbal products are formulated using nano-carriers. However, one of the concerns using these carriers is alteration in morphology of cell by using such particles or droplets.

Methods: In the present work, Giemsa staining was used to study the morphological changes on mammalian Neuro-2a cell line upon treatment with prepared capsaicin loaded nanoemulsion (Caps-NE), baclofen loaded poly(lactic-co-glycolic acid) nanoparticles (BcfPLGA-NPs) and lamotrigine loaded poly(lactic-co-glycolic acid) nanoparticles (Ltg-PLGA-NPs). Caps-NE, Bcf-PLGA-NPs and LtgPLGA-NPs along with their respective aqueous drug counterparts were examined for visible morphological changes at their corresponding reported maximum plasma concentration (Cmax) and 10xCmax values.

Results: Microscopic images showed that drug loaded nanoemulsion/nanoparticles showed comparable cell viability and exhibited lesser cytotoxicity than the available aqueous drug forms at both the concentrations.

Conclusion: The prepared formulations can used as potentially safer option as compared to their aqueous counterparts.

Keywords: Capsaicin, Baclofen, Lamotrigine, nanoparticles, nanoemulsions, Giemsa Staining.

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Article Details

(E-pub Ahead of Print)
DOI: 10.2174/2210681210999200508084714
Price: $95