Background: Nano-formulations of various drugs and natural compounds are becoming
popular day by day. A number of cosmetic, medicinal and herbal products are formulated using
nano-carriers. However, one of the concerns using these carriers is the alteration in morphology of
cell by using such particles or droplets.
Methods: In the present work, Giemsa staining was used to study the morphological changes on
mammalian Neuro-2a cell line upon treatment with prepared capsaicin loaded nanoemulsion
(Caps-NE), baclofen loaded poly(lactic-co-glycolic acid) nanoparticles (Bcf-PLGA-NPs) and
lamotrigine loaded poly(lactic-co-glycolic acid) nanoparticles (Ltg-PLGA-NPs). Caps-NE, Bcf-
PLGA-NPs and Ltg-PLGA-NPs, along with their respective aqueous drug counterparts, were
examined for visible morphological changes at their corresponding reported maximum plasma
concentration (Cmax) and 10xCmax values.
Results: Microscopic images showed that drug loaded nanoemulsion/nanoparticles showed
comparable cell viability and exhibited lesser cytotoxicity than the available aqueous drug forms at
both the concentrations.
Conclusion: The prepared formulations can be used as potentially safer option as compared to
their aqueous counterparts.