Background: Nano-formulations of various drugs and natural compounds are becoming popular day by day. Number of
cosmetic, medicinal and herbal products are formulated using nano-carriers. However, one of the concerns using these carriers is
alteration in morphology of cell by using such particles or droplets.
Methods: In the present work, Giemsa staining was used to study the morphological changes on mammalian Neuro-2a cell line upon
treatment with prepared capsaicin loaded nanoemulsion (Caps-NE), baclofen loaded poly(lactic-co-glycolic acid) nanoparticles (BcfPLGA-NPs) and lamotrigine loaded poly(lactic-co-glycolic acid) nanoparticles (Ltg-PLGA-NPs). Caps-NE, Bcf-PLGA-NPs and LtgPLGA-NPs along with their respective aqueous drug counterparts were examined for visible morphological changes at their
corresponding reported maximum plasma concentration (Cmax) and 10xCmax values.
Results: Microscopic images showed that drug loaded nanoemulsion/nanoparticles showed comparable cell viability and exhibited
lesser cytotoxicity than the available aqueous drug forms at both the concentrations.
Conclusion: The prepared formulations can used as potentially safer option as compared to their aqueous counterparts.