Background: A reduced concentration of adiponectin is considered as an independent factor
of the risk of inducing endometrial cancer. Cisplatin is a drug used in the therapy of this type of neoplasm.
However, knowledge of the effects of cisplatin on the adiponectin level is still limited.
Objective: The purpose of this study was to assess the impact of cisplatin depending on the concentration
and time of exposition of the cells to the drug on the adiponectin level in the endometrial cancer
Methods: Cells of endometrial cancer cell line Ishikawa were exposed for 12,24 and 48 hour periods to
cisplatin with the following concentrations: 2.5μM, 5μM, 10μM. The changes in the expression profile
of adiponectin were compared to the RtqPCR reaction and ELISA test. The STATISTICA 13.0 PL
program was used for statistical analysis (p<0.05).
Results: In the culture without the drug, the concentration of adiponectin was statistically lower than in
the cell culture incubated with the drug. Changes on the mRNA level seem to be more specific than on
the protein level, although in both cases, the same trend in the expression changes was noted.
Discussion: The longer the time of exposition of the cells to the drug, the expression of mRNA, and
the adiponectin protein increased. Changes in the expression profile were characterized statistically
Conclusion: Cisplatin, in a noticeable way, changes the expression profile of adiponectin. Molecular
analysis indicated that in the case of endometrial cancer therapy should be implemented with a concentration
of no less than 5 μM.